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Literature summary for 2.4.2.8 extracted from
- Purification and properties of hypoxanthine phosphoribosyltransferase from Streptomyces cyanogenus (1980), Agric. Biol. Chem., 44, 1999-2006.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| 5-phospho-alpha-D-ribose 1-diphosphate stabilizes against heat inactivation | Streptomyces cyanogenus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Streptomyces cyanogenus |  |
| EDTA | - |
Streptomyces cyanogenus | |
| GDP | - |
Streptomyces cyanogenus | |
| GMP | - |
Streptomyces cyanogenus | |
| GTP | - |
Streptomyces cyanogenus | |
| IDP | - |
Streptomyces cyanogenus | |
| IMP | - |
Streptomyces cyanogenus | |
| purine nucleotides | - |
Streptomyces cyanogenus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0016 | - |
hypoxanthine | - |
Streptomyces cyanogenus | |
| 0.0027 | - |
guanine | - |
Streptomyces cyanogenus | |
| 0.063 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Streptomyces cyanogenus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activating effect of Mn2+ is higher than that of Mg2+ | Streptomyces cyanogenus | |
| Mn2+ | activating effect of Mn2+ is higher than that of Mg2+ | Streptomyces cyanogenus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18000 | - |
8 * 18000, SDS-PAGE | Streptomyces cyanogenus |
| 150000 | - |
gel filtration | Streptomyces cyanogenus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces cyanogenus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces cyanogenus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 66.5 | - |
purified enzyme | Streptomyces cyanogenus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 50 mM Tris-HCl, pH 7.8, 1 M KCl, 10 mM MgCl2, stable at least 1 month | Streptomyces cyanogenus |
| 4°C, 50 mM Tris-HCl, pH 7.8, complete loss of activity after 4 days | Streptomyces cyanogenus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Streptomyces cyanogenus | GMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Streptomyces cyanogenus | IMP + diphosphate | - |
? | |
| additional information | no substrate: adenine | Streptomyces cyanogenus | ? | - |
? | |
| xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | poor substrate | Streptomyces cyanogenus | XMP + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 18000, SDS-PAGE | Streptomyces cyanogenus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
pH 7.4, stable | Streptomyces cyanogenus |
| 40 | - |
pH 7.4, gradual decrease of activity | Streptomyces cyanogenus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 4.4 | Streptomyces cyanogenus |
| 8.5 | - |
- |
Streptomyces cyanogenus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 9.7 | - |
Streptomyces cyanogenus |
| 6.3 | 9.7 | pH 6.3: about 50% of activity maximum, pH 9.7: about 75% of activity maximum | Streptomyces cyanogenus |
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