Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetate | slightly activating | Salmonella enterica subsp. enterica serovar Typhimurium | |
acetate | slightly activating | Escherichia coli |
General Stability | Organism |
---|---|
5-phospho-alpha-D-ribose 1-diphosphate stabilizes | Salmonella enterica subsp. enterica serovar Typhimurium |
5-phospho-alpha-D-ribose 1-diphosphate stabilizes | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-aminopurine nucleotides | - |
Escherichia coli | |
6-aminopurine nucleotides | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
6-Hydroxypurine nucleotides | - |
Escherichia coli | |
6-Hydroxypurine nucleotides | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Ba2+ | strong | Escherichia coli | |
Ba2+ | strong | Salmonella enterica subsp. enterica serovar Typhimurium | |
Ca2+ | strong | Escherichia coli | |
Ca2+ | strong | Salmonella enterica subsp. enterica serovar Typhimurium | |
nucleotides | all free 5'-nucleotides are inhibitory, 6-OH purine nucleotides are most inhibitory, while 6-NH2 purine only at high concentrations | Escherichia coli | |
nucleotides | all free 5'-nucleotides are inhibitory, 6-OH purine nucleotides are most inhibitory, while 6-NH2 purine only at high concentrations | Salmonella enterica subsp. enterica serovar Typhimurium | |
ppGpp | strong | Escherichia coli | |
ppGpp | strong | Salmonella enterica subsp. enterica serovar Typhimurium | |
Zn2+ | strong | Escherichia coli | |
Zn2+ | strong | Salmonella enterica subsp. enterica serovar Typhimurium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 16020 | - |
membrane | - |
Escherichia coli | 16020 | - |
periplasm | - |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
periplasm | - |
Escherichia coli | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolute requirement for Mg2+ or Mn2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
Mg2+ | absolute requirement for Mg2+ or Mn2+ | Escherichia coli | |
Mn2+ | absolute requirement for Mg2+ or Mn2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
Mn2+ | absolute requirement for Mg2+ or Mn2+ | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | - |
phosphoribosyltransferases for hypoxanthine and guanine are separate enzymes | - |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
strain LT-2 | - |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
phosphoribosyltransferases for hypoxanthine and guanine are separate enzymes | - |
Purification (Comment) | Organism |
---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
additional information | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, dilute aqueous purified enzyme preparation, a few weeks | Salmonella enterica subsp. enterica serovar Typhimurium |
-20°C, dilute aqueous purified enzyme preparation, a few weeks | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | phosphoribosyltransferases for hypoxanthine and guanine are separate enzymes, and catalyse the 2 reactions with different specificities | Salmonella enterica subsp. enterica serovar Typhimurium | GMP + diphosphate | - |
? | |
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | phosphoribosyltransferases for hypoxanthine and guanine are separate enzymes, and catalyse the 2 reactions with different specificities | Escherichia coli | GMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | phosphoribosyltransferases for hypoxanthine and guanine are separate enzymes, and catalyse the 2 reactions with different specificities | Salmonella enterica subsp. enterica serovar Typhimurium | IMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | phosphoribosyltransferases for hypoxanthine and guanine are separate enzymes, and catalyse the 2 reactions with different specificities | Escherichia coli | IMP + diphosphate | - |
? | |
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | XMP + diphosphate | - |
? | |
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Escherichia coli | XMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GPRT | phosphoribosyltransferases for hypoxanthine and guanine are separate enzymes | Salmonella enterica subsp. enterica serovar Typhimurium |
GPRT | phosphoribosyltransferases for hypoxanthine and guanine are separate enzymes | Escherichia coli |
HPRT | phosphoribosyltransferases for hypoxanthine and guanine are separate enzymes | Salmonella enterica subsp. enterica serovar Typhimurium |
HPRT | phosphoribosyltransferases for hypoxanthine and guanine are separate enzymes | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
guanine phosphoribosyltransferase | Salmonella enterica subsp. enterica serovar Typhimurium |
7.9 | - |
guanine phosphoribosyltransferase | Escherichia coli |
8.4 | - |
hypoxanthine phosphoribosyltransferase | Salmonella enterica subsp. enterica serovar Typhimurium |
8.4 | - |
hypoxanthine phosphoribosyltransferase | Escherichia coli |
8.6 | - |
substrate xanthine | Salmonella enterica subsp. enterica serovar Typhimurium |
8.6 | - |
substrate xanthine | Escherichia coli |