Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.2.8 extracted from

  • Montero, C.; Llorente, P.
    Artemia purine phosphoribosyltransferases. Purification and characterization (1991), Biochem. J., 275, 327-334.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.2.8

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
 Artemia sp.
Ca2+ strong Artemia sp.
GMP mixed inhibition Artemia sp.
guanine substrate inhibition Artemia sp.
hypoxanthine substrate inhibition Artemia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 guanine below Artemia sp.
0.001
-
 hypoxanthine below Artemia sp.
0.015
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Artemia sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Artemia sp. 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Artemia sp.
Mg2+ maximal activity at: 1 mM Artemia sp.
Mg2+ Km: 0.04-0.05 mM Artemia sp.
Mn2+ can replace Mg2+ Artemia sp.
Mn2+ Km: 0.015-0.020 mM Artemia sp.
Zn2+ activates, can replace Mg2+, Km: 0.066-0.075 mM Artemia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
 4 * 19000, SDS-PAGE Artemia sp.
66000
-
 gel filtration Artemia sp.

Organism

Organism UniProt Comment Textmining
Artemia sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Artemia sp.

Source Tissue

Source Tissue Comment Organism Textmining
cyst
-
 Artemia sp.
-
nauplius
-
 Artemia sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.65
-
 purified enzyme Artemia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Artemia sp. GMP + diphosphate
-
 ?
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Artemia sp. IMP + diphosphate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 19000, SDS-PAGE Artemia sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Artemia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 2 buffer-independent pH-optima: pH 7.0 and pH 9.5 Artemia sp.
9.5
-
 2 buffer-independent pH-optima: pH 7.0 and pH 9.5 Artemia sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.01
-
 guanine
-
 Artemia sp.
0.08
-
 hypoxanthine
-
 Artemia sp.