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Literature summary for 2.4.2.7 extracted from

  • Garcia-Huertas, P.; Mejia-Jaramillo, A.M.; Gonzalez, L.; Triana-Chavez, O.
    Transcriptome and functional genomics reveal the participation of adenine phosphoribosyltransferase in Trypanosoma cruzi resistance to benznidazole (2017), J. Cell. Biochem., 118, 1936-1945 .
    View publication on PubMed
Search for more literature for 2.4.2.7

Cloned(Commentary)

Cloned (Comment) Organism
transcriptome sequencing and analysis, recombinant overexpression of GFP-tagged enzyme in Trypanosoma cruzi parasites, subcloning in Escherichia coli strain DH5alpha, quantitative RT-PCR expression analysis Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
benznidazole transcriptome and functional genomics reveal the participation of adenine phosphoribosyltransferase in Trypanosoma cruzi resistance to benznidazole Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
adenine + 5-phospho-alpha-D-ribose 1-diphosphate Trypanosoma cruzi
-
 AMP + diphosphate
-
 r
adenine + 5-phospho-alpha-D-ribose 1-diphosphate Trypanosoma cruzi MHOM
-
 AMP + diphosphate
-
 r
adenine + 5-phospho-alpha-D-ribose 1-diphosphate Trypanosoma cruzi DA (TcI)
-
 AMP + diphosphate
-
 r
adenine + 5-phospho-alpha-D-ribose 1-diphosphate Trypanosoma cruzi COL
-
 AMP + diphosphate
-
 r

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
-
-
-
Trypanosoma cruzi COL
-
-
-
Trypanosoma cruzi DA (TcI)
-
-
-
Trypanosoma cruzi MHOM
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
epimastigote
-
 Trypanosoma cruzi
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Trypanosoma cruzi AMP + diphosphate
-
 r
adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Trypanosoma cruzi MHOM AMP + diphosphate
-
 r
adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Trypanosoma cruzi DA (TcI) AMP + diphosphate
-
 r
adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Trypanosoma cruzi COL AMP + diphosphate
-
 r

Subunits

Subunits Comment Organism
? x * 25860, recombinant enzyme, SDS-PAGE Trypanosoma cruzi

Synonyms

Synonyms Comment Organism
APRT
-
 Trypanosoma cruzi

pI Value

Organism Comment pI Value Maximum pI Value
Trypanosoma cruzi recombinant enzyme, isoelectric focusing
-
 7.32

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0327
-
 DA strain, pH and temperature not specified in the publication Trypanosoma cruzi benznidazole

Expression

Organism Comment Expression
Trypanosoma cruzi the gene encoding adenine phosphoribosyltransferase (APRT) is downregulated by 5.2fold in the parasites resistant against benznidazole (Bz) and nifurtimox (Nfx), and its expression profile is confirmed by 2D electrophoresis analysis down
Trypanosoma cruzi overexpression of APRT (pTREX-APRT) in benznidazole sensitive parasites, relative to the control parasites transfected with GFP (pTREX-GFP), confirmed by quantitative RT-PCR and northern blot analysis. 15.6fold and 4.6fold overexpressions are detected for the two different techniques, respectively up

General Information

General Information Comment Organism
additional information Trypanosoma cruzi regulation is mainly posttranscriptional Trypanosoma cruzi
physiological function expression and role of adenine phosphoribosyltransferase (APRT) in Trypanosoma cruzi resistance to benznidazole (Bz), overview Trypanosoma cruzi