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Literature summary for 2.4.2.7 extracted from

  • Sinitsyna, E.; Timofeev, V.; Tuzova, E.; Kostromina, M.; Murav'eva, T.; Esipov, R.; Kuranova, I.
    Crystallization and preliminary X-ray diffraction study of recombinant adenine phosphoribosyltransferase from the thermophilic bacterium Thermus thermophilus strain HB27 (2017), Crystallogr. Rep., 62, 580-583 .
No PubMed abstract available
Search for more literature for 2.4.2.7

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, hanging drop vapor diffusion technique, mixing of 12.5 mg/ml protein in 0.02 M Tris-HCl buffer, pH 8.0, 50 mM NaCl, 5% glycerol, 0.04% NaN3, and 5 mM AMP, with reservoir solution composed of 25% w/v PEG 3350, 0.1 M HEPES, pH 7.0, 0.5 M NaCl, and 0.04% NaN3, X-ray diffraction structure determination and analysis at 2.60 A resolution Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
adenine + 5-phospho-alpha-D-ribose 1-diphosphate Thermus thermophilus
-
 AMP + diphosphate
-
 r
adenine + 5-phospho-alpha-D-ribose 1-diphosphate Thermus thermophilus DSM 7039
-
 AMP + diphosphate
-
 r
adenine + 5-phospho-alpha-D-ribose 1-diphosphate Thermus thermophilus ATCC BAA-163
-
 AMP + diphosphate
-
 r

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q72I82
-
-
Thermus thermophilus ATCC BAA-163 Q72I82
-
-
Thermus thermophilus DSM 7039 Q72I82
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by heat treatment for 10 min at 65°C, desalting gel filtration, anion exchange and hydrophobic interaction chromatography, ultrafiltration, and gel filtration Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-diaminpurine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus 2,6-diaminpurine 5'-phosphoribosyl nucleotide + diphosphate
-
 r
2,6-diaminpurine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus DSM 7039 2,6-diaminpurine 5'-phosphoribosyl nucleotide + diphosphate
-
 r
2,6-diaminpurine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus ATCC BAA-163 2,6-diaminpurine 5'-phosphoribosyl nucleotide + diphosphate
-
 r
2-chloroadenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus 2-chloro-AMP + diphosphate
-
 r
2-chloroadenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus DSM 7039 2-chloro-AMP + diphosphate
-
 r
2-chloroadenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus ATCC BAA-163 2-chloro-AMP + diphosphate
-
 r
2-fluoroadenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus 2-fluoro-AMP + diphosphate
-
 r
2-fluoroadenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus DSM 7039 2-fluoro-AMP + diphosphate
-
 r
2-fluoroadenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus ATCC BAA-163 2-fluoro-AMP + diphosphate
-
 r
2-methoxyadenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus 2-methoxyadenosine 5'-monophosphate + diphosphate
-
 r
adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus AMP + diphosphate
-
 r
adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus DSM 7039 AMP + diphosphate
-
 r
adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus ATCC BAA-163 AMP + diphosphate
-
 r
additional information not only adenine but also a number of its derivatives, including 2-chloro- and 2-fluoroadenine, 2-methoxyadenine, N1-methoxyadenine, and 2,6-diaminpurine, are substrates of the enzyme Thermus thermophilus ?
-
-
additional information not only adenine but also a number of its derivatives, including 2-chloro- and 2-fluoroadenine, 2-methoxyadenine, N1-methoxyadenine, and 2,6-diaminpurine, are substrates of the enzyme Thermus thermophilus DSM 7039 ?
-
-
additional information not only adenine but also a number of its derivatives, including 2-chloro- and 2-fluoroadenine, 2-methoxyadenine, N1-methoxyadenine, and 2,6-diaminpurine, are substrates of the enzyme Thermus thermophilus ATCC BAA-163 ?
-
-
N1-methoxyadenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus N1-methoxyadenosine 5'-monophosphate + diphosphate
-
 r

Synonyms

Synonyms Comment Organism
APRT
-
 Thermus thermophilus
TthAPRT
-
 Thermus thermophilus
TT_C1250 locus name Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
73
-
 recombinant enzyme Thermus thermophilus

General Information

General Information Comment Organism
physiological function adenine phosphoribosyltransferase (APRT) belongs to the type I phosphoribosyltransferases and catalyzes the formation of adenosine monophosphate via transfer of the 5-phosphoribosyl group from phosphoribosyl diphosphate to the nitrogen atom N9 of the adenine base Thermus thermophilus