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Literature summary for 2.4.2.7 extracted from

  • Zhang, X.; Chen, Y.; Lin, X.; Hong, X.; Zhu, Y.; Li, W.; He, W.; An, F.; Guo, H.
    Adenine phosphoribosyl transferase 1 is a key enzyme catalyzing cytokinin conversion from nucleobases to nucleotides in Arabidopsis (2013), Mol. Plant, 6, 1661-1672.
    View publication on PubMed
Search for more literature for 2.4.2.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
G168E the mutant of isoform APT1 shows reduced activity with zeatin (about 14%) and adenine (about 28%), respectively, compared to the wild type enzyme Arabidopsis thaliana
G195D the mutant of isoform APT1 shows nearly no activity with zeatin and adenine, respectively, compared to the wild type enzyme Arabidopsis thaliana
G196R the mutant of isoform APT1 shows nearly no activity with zeatin and adenine, respectively, compared to the wild type enzyme Arabidopsis thaliana
L96F the mutant of isoform APT1 shows reduced activity with zeatin (about 11%) and adenine (about 8%), respectively, compared to the wild type enzyme Arabidopsis thaliana
P85T the mutant of isoform APT1 shows nearly no activity with zeatin and adenine, respectively, compared to the wild type enzyme Arabidopsis thaliana
R149K the mutant of isoform APT1 shows no activity with zeatin and adenine, respectively, compared to the wild type enzyme Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P31166 ecotype Col-0
-
Arabidopsis thaliana Q42563 ecotype Col-0
-
Arabidopsis thaliana Q9LFP0 ecotype Col-0
-
Arabidopsis thaliana Q9SU38 ecotype Col-0
-

Purification (Commentary)

Purification (Comment) Organism
Ni affinity column chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenine + 5-phospho-alpha-D-ribose 1-diphosphate isoform APT1 shows 100% activity with adenine Arabidopsis thaliana AMP + diphosphate
-
 ?
additional information isoform APT2 is nearly inactive with adenine and zeatin Arabidopsis thaliana ?
-
 ?
additional information isoform APT4 is nearly inactive with adenine Arabidopsis thaliana ?
-
 ?
additional information isoform APT5 is nearly inactive with adenine Arabidopsis thaliana ?
-
 ?
zeatin + 5-phospho-alpha-D-ribose 1-diphosphate isoform APT4 shows about 38% activity with zeatin Arabidopsis thaliana zeatin riboside 5'-phosphate + diphosphate
-
 ?
zeatin + 5-phospho-alpha-D-ribose 1-diphosphate isoform APT5 shows 100% activity with zeatin Arabidopsis thaliana zeatin riboside 5'-phosphate + diphosphate
-
 ?
zeatin + 5-phospho-alpha-D-ribose 1-diphosphate isoforms APT1 shows 100% activity with zeatin Arabidopsis thaliana zeatin riboside 5'-phosphate + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
adenine phosphoribosyl transferase
-
 Arabidopsis thaliana
APRT
-
 Arabidopsis thaliana
APT1 isoform Arabidopsis thaliana
APT2 isoform Arabidopsis thaliana
APT4 isoform Arabidopsis thaliana
APT5 isoform Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction loss of enzyme activity leads to excess accumulation of cytokinin bases, thus evoking myriad cytokinin-regulated responses, such as delayed leaf senescence, anthocyanin accumulation, and downstream gene expression Arabidopsis thaliana
metabolism adenine phosphoribosyl transferase 1 is a key metabolic enzyme participating in the cytokinin inactivation by phosphoribosylation Arabidopsis thaliana