Cloned (Comment) | Organism |
---|---|
cloning and DNA sequence determination, expression in Escherichia coli BL21 cells as C-terminally His-tagged protein | Giardia intestinalis |
Protein Variants | Comment | Organism |
---|---|---|
F25W | site-directed mutagenesis, tryptophan at the adenine binding site, kinetic constants similar to the wild-type | Giardia intestinalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | competitive against 5-phospho-alpha-D-ribose 1-diphosphate; competitive against adenine | Giardia intestinalis | |
diphosphate | noncompetitive against 5-phospho-alpha-D-ribose 1-diphosphate | Giardia intestinalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Giardia intestinalis | |
0.0042 | - |
adenine | - |
Giardia intestinalis | |
0.087 | - |
AMP | - |
Giardia intestinalis | |
0.143 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Giardia intestinalis | |
0.45 | - |
diphosphate | - |
Giardia intestinalis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Giardia intestinalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Giardia intestinalis | Q967M2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli | Giardia intestinalis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate | forward reaction follows a random bi-bi mechanism | Giardia intestinalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
wild-type and mutant | Giardia intestinalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Giardia intestinalis | AMP + diphosphate | ordered substrate binding in the reverse reaction with AMP bound first followed by diphosphate | r |
Subunits | Comment | Organism |
---|---|---|
? | x * 20000-20370, recombinant His-tagged enzyme, SDS-PAGE and DNA sequence determination | Giardia intestinalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0095 | - |
diphosphate | - |
Giardia intestinalis | |
0.0095 | - |
AMP | - |
Giardia intestinalis | |
2.8 | - |
adenine | - |
Giardia intestinalis | |
2.8 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Giardia intestinalis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
AMP | competitive versus 5-phosphoribose 1-diphosphate | Giardia intestinalis | |
1.79 | - |
diphosphate | versus 5-phosphoribose 1-diphosphate | Giardia intestinalis | |
23.5 | - |
AMP | competitive versus adenine | Giardia intestinalis |