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Literature summary for 2.4.2.7 extracted from

  • Sarver, A.E.; Wang, C.C.
    The adenine phosphoribosyltransferase from Giardia lamblia has a unique reaction mechanism and unusual substrate binding properties (2002), J. Biol. Chem., 277, 39973-39980.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloning and DNA sequence determination, expression in Escherichia coli BL21 cells as C-terminally His-tagged protein Giardia intestinalis

Protein Variants

Protein Variants Comment Organism
F25W site-directed mutagenesis, tryptophan at the adenine binding site, kinetic constants similar to the wild-type Giardia intestinalis

Inhibitors

Inhibitors Comment Organism Structure
AMP competitive against 5-phospho-alpha-D-ribose 1-diphosphate; competitive against adenine Giardia intestinalis
diphosphate noncompetitive against 5-phospho-alpha-D-ribose 1-diphosphate Giardia intestinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Giardia intestinalis
0.0042
-
adenine
-
Giardia intestinalis
0.087
-
AMP
-
Giardia intestinalis
0.143
-
5-phospho-alpha-D-ribose 1-diphosphate
-
Giardia intestinalis
0.45
-
diphosphate
-
Giardia intestinalis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Giardia intestinalis

Organism

Organism UniProt Comment Textmining
Giardia intestinalis Q967M2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli Giardia intestinalis

Reaction

Reaction Comment Organism Reaction ID
AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate forward reaction follows a random bi-bi mechanism Giardia intestinalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
wild-type and mutant Giardia intestinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Giardia intestinalis AMP + diphosphate ordered substrate binding in the reverse reaction with AMP bound first followed by diphosphate r

Subunits

Subunits Comment Organism
? x * 20000-20370, recombinant His-tagged enzyme, SDS-PAGE and DNA sequence determination Giardia intestinalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0095
-
diphosphate
-
Giardia intestinalis
0.0095
-
AMP
-
Giardia intestinalis
2.8
-
adenine
-
Giardia intestinalis
2.8
-
5-phospho-alpha-D-ribose 1-diphosphate
-
Giardia intestinalis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.018
-
AMP competitive versus 5-phosphoribose 1-diphosphate Giardia intestinalis
1.79
-
diphosphate versus 5-phosphoribose 1-diphosphate Giardia intestinalis
23.5
-
AMP competitive versus adenine Giardia intestinalis