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Literature summary for 2.4.2.7 extracted from
- Artemia purine phosphoribosyltransferases. Purification and characterization (1991), Biochem. J., 275, 327-334.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | competitive against 5-phospho-alpha-D-ribose 1-diphosphate | Artemia sp. |  |
| Ba2+ | - |
Artemia sp. | |
| Ca2+ | strong | Artemia sp. | |
| guanine | noncompetitive against 5-phosphoribose 1-diphosphate | Artemia sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
adenine | - |
Artemia sp. | |
| 0.03 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Artemia sp. | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Artemia sp. | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | inhibition | Artemia sp. | |
| Mg2+ | required | Artemia sp. | |
| Mg2+ | 0.15-0.18 mM | Artemia sp. | |
| Mn2+ | Km: 0.04-0.05 | Artemia sp. | |
| Mn2+ | activity depends on presence of divalent cations, Mn2+ or Mg2+ activates | Artemia sp. | |
| Zn2+ | activates, Km: 0.045-0.05 mM | Artemia sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15000 | - |
2 * 15000, SDS-PAGE | Artemia sp. |
| 28000 | - |
gel filtration | Artemia sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Artemia sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Artemia sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cyst | - |
Artemia sp. | - |
| nauplius | - |
Artemia sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.2 | - |
purified enzyme | Artemia sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Artemia sp. | AMP + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 15000, SDS-PAGE | Artemia sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Artemia sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | - |
Artemia sp. |
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