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Literature summary for 2.4.2.7 extracted from

  • Nagy, M.; Ribet, A.M.
    Purification and comparative study of adenine and guanine phosphoribosyltransferases from Schizosaccharomyces pombe (1977), Eur. J. Biochem., 77, 77-85.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
Schizosaccharomyces pombe
AMP
-
Schizosaccharomyces pombe
ATP
-
Schizosaccharomyces pombe
CTP
-
Schizosaccharomyces pombe
dAMP
-
Schizosaccharomyces pombe
dATP
-
Schizosaccharomyces pombe
diphosphate at 20 mM Schizosaccharomyces pombe
GDP slightly at 5 mM Schizosaccharomyces pombe
GMP slightly at 5 mM Schizosaccharomyces pombe
GTP slightly at 5 mM Schizosaccharomyces pombe
Mg2+ inhibitory effects are noncompetitive against 5-phosphoribose 1-diphosphate Schizosaccharomyces pombe
additional information mechanism of product inhibition; no inhibition by GMP, XMP, UMP, CMP, and CDP Schizosaccharomyces pombe
UDP slightly Schizosaccharomyces pombe

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
5-phospho-alpha-D-ribose 1-diphosphate
-
Schizosaccharomyces pombe
0.069
-
adenine
-
Schizosaccharomyces pombe

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolute requirement Schizosaccharomyces pombe
Mg2+ maximal activity at 1-2 mM Schizosaccharomyces pombe

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
gel filtration, 2 forms: 44000 Da and 50000 Da, in presence of 5 mM MgCl2 dissociation into a single form of apparent MW 30000 Schizosaccharomyces pombe
50000
-
gel filtration, 2 forms: 44000 Da and 50000 Da, in presence of 5 mM MgCl2 dissociation into a single form of apparent MW 30000 Schizosaccharomyces pombe

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Schizosaccharomyces pombe

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18
-
-
Schizosaccharomyces pombe

Storage Stability

Storage Stability Organism
-20°C, purified enzyme, 6 mM 5-phospho-alpha-D-ribose 1-diphosphate, 2 mM MgCl2, stable for at least a month Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Schizosaccharomyces pombe AMP + diphosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Schizosaccharomyces pombe

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
10 min, stable in presence of 10 mM AMP Schizosaccharomyces pombe

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
two zones of pH-optima at pH 7.5 and pH 8.5 Schizosaccharomyces pombe
8.5
-
two zones of pH-optima at pH 7.5 and 8.5 Schizosaccharomyces pombe