Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Schizosaccharomyces pombe | |
AMP | - |
Schizosaccharomyces pombe | |
ATP | - |
Schizosaccharomyces pombe | |
CTP | - |
Schizosaccharomyces pombe | |
dAMP | - |
Schizosaccharomyces pombe | |
dATP | - |
Schizosaccharomyces pombe | |
diphosphate | at 20 mM | Schizosaccharomyces pombe | |
GDP | slightly at 5 mM | Schizosaccharomyces pombe | |
GMP | slightly at 5 mM | Schizosaccharomyces pombe | |
GTP | slightly at 5 mM | Schizosaccharomyces pombe | |
Mg2+ | inhibitory effects are noncompetitive against 5-phosphoribose 1-diphosphate | Schizosaccharomyces pombe | |
additional information | mechanism of product inhibition; no inhibition by GMP, XMP, UMP, CMP, and CDP | Schizosaccharomyces pombe | |
UDP | slightly | Schizosaccharomyces pombe |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Schizosaccharomyces pombe | |
0.069 | - |
adenine | - |
Schizosaccharomyces pombe |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolute requirement | Schizosaccharomyces pombe | |
Mg2+ | maximal activity at 1-2 mM | Schizosaccharomyces pombe |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
gel filtration, 2 forms: 44000 Da and 50000 Da, in presence of 5 mM MgCl2 dissociation into a single form of apparent MW 30000 | Schizosaccharomyces pombe |
50000 | - |
gel filtration, 2 forms: 44000 Da and 50000 Da, in presence of 5 mM MgCl2 dissociation into a single form of apparent MW 30000 | Schizosaccharomyces pombe |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Schizosaccharomyces pombe |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18 | - |
- |
Schizosaccharomyces pombe |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme, 6 mM 5-phospho-alpha-D-ribose 1-diphosphate, 2 mM MgCl2, stable for at least a month | Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Schizosaccharomyces pombe | AMP + diphosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Schizosaccharomyces pombe |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
10 min, stable in presence of 10 mM AMP | Schizosaccharomyces pombe |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
two zones of pH-optima at pH 7.5 and pH 8.5 | Schizosaccharomyces pombe |
8.5 | - |
two zones of pH-optima at pH 7.5 and 8.5 | Schizosaccharomyces pombe |