Activating Compound | Comment | Organism | Structure |
---|---|---|---|
nucleotides | effect is strongly influenced by pH, inhibition at pH 7.1: ATP, GMP, activation at pH 7.1: GTP, UMP, UTP, CMP, CTP, IMP, no effect at pH 7.1: AMP, inhibition at pH 8.0: AMP, ATP, GMP, GTP, UTP, CTP, IMP, no effect at pH 8.0: UMP, CMP | Escherichia coli | |
nucleotides | effect is strongly influenced by pH, inhibition at pH 7.1: ATP, GMP, activation at pH 7.1: GTP, UMP, UTP, CMP, CTP, IMP, no effect at pH 7.1: AMP, inhibition at pH 8.0: AMP, ATP, GMP, GTP, UTP, CTP, IMP, no effect at pH 8.0: UMP, CMP | Mycoplasma mycoides |
General Stability | Organism |
---|---|
5-phospho-alpha-D-ribose 1-diphosphate stabilizes Mycoplasma mycoides enzyme markedly | Mycoplasma mycoides |
bovine serum albumin or adenine stabilizes Mycoplasma mycoides enzyme slightly | Mycoplasma mycoides |
freezing and thawing have little effect on E. coli enzyme, but inactivate Mycoplasma mycoides enzyme | Escherichia coli |
freezing and thawing have little effect on E. coli enzyme, but inactivate Mycoplasma mycoides enzyme | Mycoplasma mycoides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenine | at high concentrations, at low 5-phospho-alpha-D-ribose 1-diphosphate concentration | Mycoplasma mycoides | |
AMP | competitive against 5-phospho-alpha-D-ribose 1-diphosphate | Escherichia coli | |
GMP | - |
Mycoplasma mycoides | |
nucleotides | effect is strongly influenced by pH, inhibition at pH 7.1: ATP, GMP, activation at pH 7.1: GTP, UMP, UTP, CMP, CTP, IMP, no effect at pH 7.1: AMP, inhibition at pH 8.0: AMP, ATP, GMP, GTP, UTP, CTP, IMP, no effect at pH 8.0: UMP, CMP | Escherichia coli | |
nucleotides | effect is strongly influenced by pH, inhibition at pH 7.1: ATP, GMP, activation at pH 7.1: GTP, UMP, UTP, CMP, CTP, IMP, no effect at pH 7.1: AMP, inhibition at pH 8.0: AMP, ATP, GMP, GTP, UTP, CTP, IMP, no effect at pH 8.0: UMP, CMP | Mycoplasma mycoides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
adenine | - |
Escherichia coli | |
0.01 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Mycoplasma mycoides | - |
- |
- |
Storage Stability | Organism |
---|---|
-15°C, stable for months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Escherichia coli | AMP + diphosphate | - |
? | |
adenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycoplasma mycoides | AMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | association of subunits in presence of 5-phospho-alpha-D-ribose 1-diphosphate | Mycoplasma mycoides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
37 | - |
assay at | Mycoplasma mycoides |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
40 min, 5 mM MgCl2, more than 90% loss of activity, + 0.4 mM 5-phospho-alpha-D-ribose 1-diphosphate, 30% loss of activity | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH-optimum depends on 5-phospho-alpha-D-ribose 1-diphosphate concentration | Mycoplasma mycoides |
8 | - |
around, broad, Escherichia coli | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
AMP | - |
Escherichia coli |