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Literature summary for 2.4.2.7 extracted from

  • Sin, I.L.; Finch, L.R.
    Adenine phosphoribosyltransferase in Mycoplasma mycoides and Escherichia coli (1972), J. Bacteriol., 112, 439-444.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
nucleotides effect is strongly influenced by pH, inhibition at pH 7.1: ATP, GMP, activation at pH 7.1: GTP, UMP, UTP, CMP, CTP, IMP, no effect at pH 7.1: AMP, inhibition at pH 8.0: AMP, ATP, GMP, GTP, UTP, CTP, IMP, no effect at pH 8.0: UMP, CMP Escherichia coli
nucleotides effect is strongly influenced by pH, inhibition at pH 7.1: ATP, GMP, activation at pH 7.1: GTP, UMP, UTP, CMP, CTP, IMP, no effect at pH 7.1: AMP, inhibition at pH 8.0: AMP, ATP, GMP, GTP, UTP, CTP, IMP, no effect at pH 8.0: UMP, CMP Mycoplasma mycoides

General Stability

General Stability Organism
5-phospho-alpha-D-ribose 1-diphosphate stabilizes Mycoplasma mycoides enzyme markedly Mycoplasma mycoides
bovine serum albumin or adenine stabilizes Mycoplasma mycoides enzyme slightly Mycoplasma mycoides
freezing and thawing have little effect on E. coli enzyme, but inactivate Mycoplasma mycoides enzyme Escherichia coli
freezing and thawing have little effect on E. coli enzyme, but inactivate Mycoplasma mycoides enzyme Mycoplasma mycoides

Inhibitors

Inhibitors Comment Organism Structure
adenine at high concentrations, at low 5-phospho-alpha-D-ribose 1-diphosphate concentration Mycoplasma mycoides
AMP competitive against 5-phospho-alpha-D-ribose 1-diphosphate Escherichia coli
GMP
-
Mycoplasma mycoides
nucleotides effect is strongly influenced by pH, inhibition at pH 7.1: ATP, GMP, activation at pH 7.1: GTP, UMP, UTP, CMP, CTP, IMP, no effect at pH 7.1: AMP, inhibition at pH 8.0: AMP, ATP, GMP, GTP, UTP, CTP, IMP, no effect at pH 8.0: UMP, CMP Escherichia coli
nucleotides effect is strongly influenced by pH, inhibition at pH 7.1: ATP, GMP, activation at pH 7.1: GTP, UMP, UTP, CMP, CTP, IMP, no effect at pH 7.1: AMP, inhibition at pH 8.0: AMP, ATP, GMP, GTP, UTP, CTP, IMP, no effect at pH 8.0: UMP, CMP Mycoplasma mycoides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
adenine
-
Escherichia coli
0.01
-
5-phospho-alpha-D-ribose 1-diphosphate
-
Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Mycoplasma mycoides
-
-
-

Storage Stability

Storage Stability Organism
-15°C, stable for months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Escherichia coli AMP + diphosphate
-
?
adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Mycoplasma mycoides AMP + diphosphate
-
?

Subunits

Subunits Comment Organism
More association of subunits in presence of 5-phospho-alpha-D-ribose 1-diphosphate Mycoplasma mycoides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli
37
-
assay at Mycoplasma mycoides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
40 min, 5 mM MgCl2, more than 90% loss of activity, + 0.4 mM 5-phospho-alpha-D-ribose 1-diphosphate, 30% loss of activity Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pH-optimum depends on 5-phospho-alpha-D-ribose 1-diphosphate concentration Mycoplasma mycoides
8
-
around, broad, Escherichia coli Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.026
-
AMP
-
Escherichia coli