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Literature summary for 2.4.2.7 extracted from
- Metabolism of cytokinin: phosphoribosylation of cytokinin bases by adenine phosphoribosyltransferase from wheat germ (1982), Arch. Biochem. Biophys., 214, 634-641.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation, competitive to Mg2+ | Triticum aestivum |  |
| Hg2+ | complete inhibition at 5 mM | Triticum aestivum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.074 | - |
adenine | - |
Triticum aestivum | |
| 0.11 | - |
N6-furfuryladenine | - |
Triticum aestivum | |
| 0.13 | - |
N6-(DELTA2-isopentenyl)adenine | - |
Triticum aestivum | |
| 0.154 | - |
N6-benzyladenine | - |
Triticum aestivum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Triticum aestivum | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Triticum aestivum | |
| Mg2+ | required | Triticum aestivum | |
| Mg2+ | optimal concentration: 5 mM | Triticum aestivum | |
| Mn2+ | activity depends on presence of divalent cations, Mn2+ or Mg2+ activates | Triticum aestivum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
gel filtration | Triticum aestivum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenine + 5-phospho-alpha-D-ribose 1-diphosphate | Triticum aestivum | may play a role in maintaining the supply of adequate levels of active cytokinin | AMP + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Triticum aestivum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| germ | - |
Triticum aestivum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0413 | - |
- |
Triticum aestivum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Triticum aestivum | AMP + diphosphate | - |
? | |
| adenine + 5-phospho-alpha-D-ribose 1-diphosphate | may play a role in maintaining the supply of adequate levels of active cytokinin | Triticum aestivum | AMP + diphosphate | - |
? | |
| benzyladenine + 5-phospho-alpha-D-ribose 1-diphosphate | N6-benzyladenine | Triticum aestivum | benzyladenosine 5'-phosphate + diphosphate | - |
? | |
| isopentenyladenine + 5-phospho-alpha-D-ribose 1-diphosphate | N6-(delta-isopentenyl)adenine | Triticum aestivum | isopentenyladenosine 5'-phosphate + diphosphate | - |
? | |
| additional information | no substrate: hypoxanthine,guanine | Triticum aestivum | ? | - |
? | |
| N6-furfuryladenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Triticum aestivum | N6-furfuryladenosine 5'-phosphate + diphosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Triticum aestivum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Triticum aestivum |
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