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Literature summary for 2.4.2.7 extracted from
- Adenine phosphoribosyltransferase (1978), Methods Enzymol., 51, 568-574.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Homo sapiens |  |
| AMP | - |
Homo sapiens | |
| citrate | - |
Homo sapiens | |
| GMP | - |
Homo sapiens | |
| Hg2+ | - |
Homo sapiens | |
| iodoacetate | - |
Homo sapiens | |
| Na+ | - |
Homo sapiens | |
| nucleotides | nucleotide mono-, di- and triphosphates of adenine, guanine and hypoxanthine | Homo sapiens | |
| p-hydroxymercuribenzoate | not enzyme from monkey liver | Homo sapiens | |
| SO42- | - |
Homo sapiens | |
| succinate | - |
Homo sapiens | |
| sulfhydryl reagents | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | |
| 0.14 | - |
adenine | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | exclusively | Homo sapiens | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Homo sapiens | |
| Co2+ | - |
Homo sapiens | |
| Mg2+ | required | Homo sapiens | |
| Mg2+ | absolute requirement | Homo sapiens | |
| Mg2+ | Mg2+ most effective | Homo sapiens | |
| Mg2+ | maximal activity if Mg2+ is twice the concentration of 5-phospho-alpha-D-ribose 1-diphosphate | Homo sapiens | |
| Mn2+ | - |
Homo sapiens | |
| additional information | descendring order of efficency: Mg2+, Mn2+, Ca2+, Co2+, Ni2+, Zn2+ | Homo sapiens | |
| additional information | activity absolutely depends on presence of divalent cations | Homo sapiens | |
| Ni2+ | - |
Homo sapiens | |
| Zn2+ | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
gel filtration, sucrose density gradient ultracentrifugation | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Homo sapiens | - |
| additional information | human enzyme found in all tissues with the highest specific activity in nucleated cells | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.58 | - |
purified enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,6-diaminopurine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | 2,6-diaminopurine ribotide + diphosphate | - |
? | |
| 4-amino-5-imidazolecarboxamide + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | 4-amino-5-imidazolecarboxamide ribotide + diphosphate | - |
? | |
| 6-mercaptopurine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | 6-mercaptopurine ribotide + diphosphate | - |
? | |
| adenine + 5-phospho-alpha-D-ribose 1-diphosphate | highly specific for the donor substrate | Homo sapiens | AMP + diphosphate | - |
? | |
| additional information | no substrate: adenosine | Homo sapiens | ? | - |
? | |
| additional information | no substrate: hypoxanthine,guanine | Homo sapiens | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 4.85 | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 9.5 | broad maximum | Homo sapiens |
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Release 2023.1 (January 2023)
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