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Literature summary for 2.4.2.7 extracted from
- Purification and characterization of adenine phosphoribosyltransferase from Arabidopsis thaliana (1993), Physiol. Plant., 87, 483-492.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| unstable in absence of Mg2+ and glycerol, loss 0f 70% activity during gel filtration | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenine | - |
Arabidopsis thaliana |  |
| alpha-D-5-phosphoribose 1-diphosphate | substrate inhibition | Arabidopsis thaliana | |
| AMP | - |
Arabidopsis thaliana | |
| Ba2+ | in presence of MnCl2 | Arabidopsis thaliana | |
| benzyladenine | competitive | Arabidopsis thaliana | |
| Ca2+ | in presence of MnCl2 | Arabidopsis thaliana | |
| Cd2+ | in presence of MnCl2 | Arabidopsis thaliana | |
| Hg2+ | in presence of MnCl2 | Arabidopsis thaliana | |
| isopentenyladenine | - |
Arabidopsis thaliana | |
| Mg2+ | inhibition in presence of MnCl2, activation in absence | Arabidopsis thaliana | |
| additional information | no substrate inhibition by adenine | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0045 | - |
adenine | at 37°C | Arabidopsis thaliana | |
| 0.29 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at 37°C | Arabidopsis thaliana | |
| 0.73 | - |
benzyladenine | at 37°C | Arabidopsis thaliana | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | inhibition in presence of MnCl2 | Arabidopsis thaliana | |
| Mg2+ | absolute requirement | Arabidopsis thaliana | |
| Mg2+ | enzyme requires Mn2+ or Mg2+ | Arabidopsis thaliana | |
| Mn2+ | best activating divalent cation | Arabidopsis thaliana | |
| Mn2+ | activity depends on presence of divalent cations, Mn2+ or Mg2+ activates | Arabidopsis thaliana | |
| additional information | activity absolutely depends on presence of divalent cations | Arabidopsis thaliana |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
gel filtration | Arabidopsis thaliana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Arabidopsis thaliana | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 300 | - |
purified enzyme, pH 8.8, 37°C | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-amino-4-imidazolecarboxamide + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Arabidopsis thaliana | 5-amino-4-imidazolecarboxamide ribotide + diphosphate | - |
? | |
| adenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Arabidopsis thaliana | AMP + diphosphate | - |
? | |
| benzyladenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Arabidopsis thaliana | benzyladenosine 5'-phosphate + diphosphate | - |
? | |
| isopentenyladenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Arabidopsis thaliana | isopentenyladenosine 5'-phosphate + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Arabidopsis thaliana |
| 65 | - |
- |
Arabidopsis thaliana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 58 | - |
heat denaturation, 35% ammonium sulfate protects | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
temperature dependent | Arabidopsis thaliana |
| 7.8 | - |
at 60°C | Arabidopsis thaliana |
| 8.8 | - |
at 37°C | Arabidopsis thaliana |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.96 | - |
5-phosphoribose 1-diphosphate | at 37°C | Arabidopsis thaliana | |
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