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Literature summary for 2.4.2.60 extracted from

  • Garcia, A.; Dyszy, F.; Munte, C.; Demarco, R.; Beltramini, L.; Oliva, G.; Costa-Filho, A.; Araujo, A.
    THI1, a protein involved in the biosynthesis of thiamin in Arabidopsis thaliana Structural analysis of THI1(A140V) mutant (2014), Biochim. Biophys. Acta, 1844, 1094-1103 .
    View publication on PubMed
Search for more literature for 2.4.2.60

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
A140V mutant contains more sulfur-containing cysteines than wild-type, and its conformational stability is affected Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
191000
-
 gel filtration, wild-type Arabidopsis thaliana
200000
-
 PAGE Arabidopsis thaliana
203000
-
 gel filtration, mutant A140V Arabidopsis thaliana
241940
-
 mass spectrometry, wild-type Arabidopsis thaliana
242290
-
 mass spectrometry, mutant A140V Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q38814
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Arabidopsis thaliana

Subunits

Subunits Comment Organism
octamer 8 * 36600, calculated Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
THI1
-
 Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
56
-
 mutant A140V, beginning of thermal denaturation Arabidopsis thaliana
60
-
 wild-type, beginning of thermal denaturation Arabidopsis thaliana