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Literature summary for 2.4.2.6 extracted from

  • Fernandez-Lucas, J.; Acebal, C.; Sinisterra, J.V.; Arroyo, M.; de la Mata, I.
    Lactobacillus reuteri 2-deoxyribosyltransferase, a novel biocatalyst for tailoring of nucleosides (2010), Appl. Environ. Microbiol., 76, 1462-1470.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information no activation observed with NH4+, K+ or Rb+ Limosilactobacillus reuteri

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Limosilactobacillus reuteri

Inhibitors

Inhibitors Comment Organism Structure
NaCl 70% activity remains at 1 M NaCl Limosilactobacillus reuteri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14
-
2'-deoxyuridine pH 6.5, 40°C Limosilactobacillus reuteri
10.79
-
cytosine pH 6.5, 40°C Limosilactobacillus reuteri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
6 * 18000, SDS-PAGE Limosilactobacillus reuteri
112700
-
sedimentation equilibrium experiments Limosilactobacillus reuteri

Organism

Organism UniProt Comment Textmining
Limosilactobacillus reuteri A5VK58
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxyadenosine + 5-chlorouracil
-
Limosilactobacillus reuteri 2'-deoxy-D-ribosyl-5-chlorouracil + adenine
-
?
2'-deoxyuridine + 2,6-diaminopurine
-
Limosilactobacillus reuteri 2,6-diaminopurine 2-deoxy-D-riboside + uracil
-
?
2'-deoxyuridine + 5-fluorocytosine
-
Limosilactobacillus reuteri 2'-deoxy-5-fluorocytidine + uracil
-
?
2'-deoxyuridine + 6-mercaptopurine
-
Limosilactobacillus reuteri 6-mercaptopurine 2-deoxy-D-ribose 5-phosphate + uracil
-
?
2'-deoxyuridine + benzimidazole
-
Limosilactobacillus reuteri benzimidazole 2-deoxy-D-ribose 5-phosphate + uracil
-
?
2'-deoxyuridine + cytosine
-
Limosilactobacillus reuteri 2'-deoxycytidine + uracil
-
?
additional information enzyme does not interchange bases between ribonucleosides, but is able to catalyze the transfer of 2'-deoxyribose between purine or pyrimidine and/or pyrimidine bases. The enzyme prefers pyrimidines to purines as substrates, dUrd being the best nucleoside donor, followed by dThd, dAdo, and dIno, whereas cytosine is the best base acceptor and hypoxanthine is the worst Limosilactobacillus reuteri ?
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 18000, SDS-PAGE Limosilactobacillus reuteri

Synonyms

Synonyms Comment Organism
2'-deoxyribosyltransferase
-
Limosilactobacillus reuteri
NDT
-
Limosilactobacillus reuteri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 45
-
Limosilactobacillus reuteri
40
-
assay at Limosilactobacillus reuteri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable up to Limosilactobacillus reuteri
64
-
melting temperature Limosilactobacillus reuteri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
483
-
2'-deoxyuridine pH 6.5, 40°C Limosilactobacillus reuteri
2083
-
cytosine pH 6.5, 40°C Limosilactobacillus reuteri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 7 broad Limosilactobacillus reuteri
6.5
-
assay at Limosilactobacillus reuteri

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 8 enzyme is completely stable in this pH interval Limosilactobacillus reuteri

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
193
-
cytosine pH 6.5, 40°C Limosilactobacillus reuteri
424
-
2'-deoxyuridine pH 6.5, 40°C Limosilactobacillus reuteri