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Literature summary for 2.4.2.6 extracted from

  • Kaminski, P.A.; Dugue, L.; Pochet, S.
    Expanding substrate specificity of nucleoside 2-deoxyribosyltransferase (2008), Nucleic Acids Symp. Ser., 52, 495-496.
    View publication on PubMed
Search for more literature for 2.4.2.6

Protein Variants

Protein Variants Comment Organism
A15C is not capable of improving 2',3'-dideoxyribose transfer reaction Limosilactobacillus fermentum
A15S Ser appears a good alternative for Thr, rate of 2',3'-dideoxyribosyl transfer from 2',3'-dideoxycytidine to adenine of the two mutants is almost identical Limosilactobacillus fermentum
A15T a single amino-acid substitution allows the conversion of the nucleoside 2'-deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2'3'-didehydro, 2',3'-dideoxyribosyltransferase activity. Specificity for the base is unchanged compared to the wild-type. The mutant displays an improved activity to the transfer of 2',3'-dideoxyribose, which is enhanced by 250fold (depending on the donors and acceptors) while the deoxyribosyl group transfer remains acceptable (30% of the wild-type) Limosilactobacillus fermentum
G9S a single amino-acid substitution allows the conversion of the nucleoside 2'-deoxyribosyltransferase activity to a nucleoside 2',3'-dideoxy- or 2'3'-didehydro, 2',3'-dideoxyribosyltransferase activity Lactobacillus leichmannii

Organism

Organism UniProt Comment Textmining
Lactobacillus leichmannii
-
-
-
Limosilactobacillus fermentum Q6YNI5
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
 wild-type, 2',3'-dideoxyadenosine and thymine as substrates Limosilactobacillus fermentum
0.01
-
 wild-type, 2',3'-dideoxycytidine and thymine as substrates Limosilactobacillus fermentum
0.03
-
 wild-type, 2',3'-dideoxyadenosine and cytosine as substrates Limosilactobacillus fermentum
0.03
-
 wild-type, 2,3'-dideoxythymidine and adenine as substrates Limosilactobacillus fermentum
0.036
-
 wild-type, 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates Limosilactobacillus fermentum
0.05
-
 wild-type, 2',3'-dideoxycytidine and hypoxanthine as substrates Limosilactobacillus fermentum
0.06
-
 wild-type, 2',3'-dideoxycytidine and adenine as substrates Limosilactobacillus fermentum
0.42
-
 mutant A15T, 2',3'-dideoxyadenosine and thymine as substrates Limosilactobacillus fermentum
0.46
-
 mutant A15T, 2'-deoxyadenosine and thymine as substrates Limosilactobacillus fermentum
0.5
-
 mutant A15T, 2,3'-dideoxythymidine and adenine as substrates Limosilactobacillus fermentum
1.1
-
 mutant A15T, 2'-deoxyadenosine and cytosine as substrates Limosilactobacillus fermentum
1.29
-
 mutant A15T, 2'-deoxycytidine and hypoxanthine as substrates Limosilactobacillus fermentum
1.35
-
 mutant A15T, 2',3'-dideoxyadenosine and cytosine as substrates Limosilactobacillus fermentum
1.47
-
 mutant A15T, 2',3'-dideoxycytidine and thymine as substrates Limosilactobacillus fermentum
1.9
-
 mutant A15T, 2'-deoxythymidine and adenine as substrates Limosilactobacillus fermentum
6.8
-
 mutant A15T, 2',3'-dideoxycytidine and hypoxanthine as substrates Limosilactobacillus fermentum
6.9
-
 wild-type, 2'-deoxycytidine and hypoxanthine as substrates Limosilactobacillus fermentum
7.1
-
 mutant A15T, 2',3'-dideoxycytidine and adenine as substrates Limosilactobacillus fermentum
7.11
-
 mutant A15T, 2'-deoxycytidine and thymine as substrates Limosilactobacillus fermentum
8.4
-
 wild-type, 2'-deoxyadenosine and thymine as substrates Limosilactobacillus fermentum
9.28
-
 mutant A15T, 2',3'didehydro-2',3'-dideoxythymidine and adenine as substrates Limosilactobacillus fermentum
9.3
-
 mutant A15T, 2'-deoxycytidine and adenine as substrates Limosilactobacillus fermentum
20.1
-
 wild-type, 2'-deoxythymidine and adenine as substrates Limosilactobacillus fermentum
32
-
 wild-type, 2'-deoxycytidine and thymine as substrates Limosilactobacillus fermentum
33.2
-
 wild-type, 2'-deoxyadenosine and cytosine as substrates Limosilactobacillus fermentum
83.7
-
 wild-type, 2'-deoxycytidine and adenine as substrates Limosilactobacillus fermentum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-dideoxyadenosine + cytosine
-
 Limosilactobacillus fermentum 2',3'-dideoxycytidine + adenine
-
 ?
2',3'-dideoxyadenosine + thymine
-
 Limosilactobacillus fermentum 2',3'-dideoxythymidine + adenine
-
 ?
2',3'-dideoxycytidine + adenine
-
 Limosilactobacillus fermentum 2',3'-dideoxyadenosine + cytosine
-
 ?
2',3'-dideoxycytidine + hypoxanthine
-
 Limosilactobacillus fermentum 2',3'-dideoxyinosine + cytosine
-
 ?
2',3'-dideoxycytidine + thymine
-
 Limosilactobacillus fermentum 2',3'-dideoxythymidine + cytosine
-
 ?
2',3'-dideoxythymidine + adenine
-
 Limosilactobacillus fermentum 2',3'-dideoxyadenosine + thymine
-
 ?
2',3'didehydro-2',3'-dideoxythymidine + adenine
-
 Limosilactobacillus fermentum 2',3'didehydro-2',3'-dideoxyadenosine + thymine
-
 ?
2'-deoxyadenosine + cytosine
-
 Limosilactobacillus fermentum 2'-deoxycytidine + adenine
-
 ?
2'-deoxyadenosine + thymine
-
 Limosilactobacillus fermentum 2'-deoxythymidine + adenine
-
 ?
2'-deoxycytidine + adenine
-
 Limosilactobacillus fermentum 2'-deoxyadenosine + cytosine
-
 ?
2'-deoxycytidine + thymine
-
 Limosilactobacillus fermentum 2'-deoxythymidine + cytosine
-
 ?
2'-deoxythymidine + adenine
-
 Limosilactobacillus fermentum 2'-deoxyadenosine + thymine
-
 ?

Synonyms

Synonyms Comment Organism
NTD
-
 Lactobacillus leichmannii
NTD
-
 Limosilactobacillus fermentum
nucleoside 2'-deoxyribosyltransferase
-
 Lactobacillus leichmannii
nucleoside 2'-deoxyribosyltransferase
-
 Limosilactobacillus fermentum