Cloned (Comment) | Organism |
---|---|
gene ugt79b1, DNA and amino acid sequence determination and analysis, expression analysis and phylogenetic analysis, recombinant expression as His-tagged protein in Escherichia coli | Arabidopsis thaliana |
recombinantly expressed as a His-tagged fusion protein | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of transposon insertion mutants of UGT79B1 | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-xylose + cyanidin 3-O-beta-D-glucoside | Arabidopsis thaliana | - |
UDP + cyanidin 3-O-beta-D-sambubioside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9LVW3 | - |
- |
Arabidopsis thaliana | Q9LVW3 | gene ugt79b1 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme converts cyanidin 3-O-glucoside to cyanidin 3-O-xylosyl(1-2)glucoside. Enzyme recognizes 3-O-glucosylated anthocyanidins/flavonols and uridine diphosphate (UDP)-xylose | Arabidopsis thaliana | ? | - |
? | |
additional information | UGT79B1 recognizes 3-O-glucosylated anthocyanidins/flavonols and UDP-xylose, but not 3,5-O-diglucosylated anthocyanidins, substrate specificity, overview. No activity with cyanidin 3-O-rhamnoside, cyanidin 3,5-O-diglucoside, cyanidin 3-O-6''-O-4-coumaroylglucoside-5-O-glucoside, cyanidin 3-O-6''-O-p-coumaroylglucoside, kaempferol 3-O-rhanoside, and kaempferol 3-O-glucoside-7-O-rhamnoside. The enzyme is absolutely specific for UDP-xylose as donar substrate, no activity with UDP-arabinose, UDP-galactose, UDP-glucose, UDP-glucuronic acid, and UDP-rhamnose | Arabidopsis thaliana | ? | - |
? | |
UDP-alpha-D-xylose + cyanidin 3-O-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside | - |
Arabidopsis thaliana | UDP + cyanidin 3-O-beta-D-xylosyl-(1->2)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside | - |
? | |
UDP-alpha-D-xylose + cyanidin 3-O-beta-D-glucoside | - |
Arabidopsis thaliana | UDP + cyanidin 3-O-beta-D-sambubioside | - |
? | |
UDP-alpha-D-xylose + delphinidin 3-O-beta-D-glucoside | - |
Arabidopsis thaliana | UDP + delphinidin 3-O-beta-D-sambubioside | - |
? | |
UDP-alpha-D-xylose + kaempferol 3-O-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside | - |
Arabidopsis thaliana | UDP + kaempferol 3-O-beta-D-xylosyl-(1->2)-[alpha-L-rhamnosyl-(1->6)]-beta-D-glucoside | - |
? | |
UDP-alpha-D-xylose + kaempferol 3-O-beta-D-glucoside | best substrate | Arabidopsis thaliana | UDP + kaempferol 3-O-beta-D-xylosyl-(1->2)-beta-D-glucoside | - |
? | |
UDP-alpha-D-xylose + pelargonidin 3-O-beta-D-glucoside | - |
Arabidopsis thaliana | UDP + pelargonidin 3-O-beta-D-sambubioside | - |
? | |
UDP-alpha-D-xylose + quercetin 3-O-beta-D-glucoside | - |
Arabidopsis thaliana | UDP + quercetin 3-O-beta-D-sambubioside | - |
? | |
UDP-D-xylose + cyanidin 3-O-(alpha-L-rhamnosyl-(1->6)-beta-D-glucoside) | - |
Arabidopsis thaliana | UDP + cyanidin 3-O-(beta-D-xylosyl-1(->2)[alpha-L-rhamnosyl-(1->6)]-beta-D-glucoside) | - |
? | |
UDP-D-xylose + cyanidin 3-O-beta-D-glucoside | - |
Arabidopsis thaliana | UDP + cyanidin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside) | - |
? | |
UDP-D-xylose + delphinidin 3-O-beta-D-glucoside | - |
Arabidopsis thaliana | UDP + delphinidin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside) | - |
? | |
UDP-D-xylose + kaempferol 3-O-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside | - |
Arabidopsis thaliana | UDP + kaempferol 3-O-(beta-D-xylosyl-(1->2)[alpha-L-rhamnosyl-(1->6)]-beta-D-glucoside) | - |
? | |
UDP-D-xylose + kaempferol 3-O-beta-D-glucoside | - |
Arabidopsis thaliana | UDP + kaempferol 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside) | - |
? | |
UDP-D-xylose + pelargonidin 3-O-beta-D-glucoside | - |
Arabidopsis thaliana | UDP + pelargonidin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside) | - |
? | |
UDP-D-xylose + quercetin 3-O-beta-D-glucoside | - |
Arabidopsis thaliana | UDP + quercetin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside) | - |
? |
Synonyms | Comment | Organism |
---|---|---|
anthocyanin 3-O-glucoside: 2''-O-xylosyltransferase | - |
Arabidopsis thaliana |
UGT79B1 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Arabidopsis thaliana |
7.5 | - |
assay at | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | flavonoid UGT phylogenetic tree, UGT79B1 belongs to a cluster in which UGTs transfer a glycosyl group to a sugar moiety of flavonoid glycosides | Arabidopsis thaliana |
malfunction | anthocyanin is drastically reduced in ugt79b1 knockout mutants | Arabidopsis thaliana |