BRENDA - Enzyme Database
show all sequences of 2.4.2.51

Two glycosyltransferases involved in anthocyanin modification delineated by transcriptome independent component analysis in Arabidopsis thaliana

Yonekura-Sakakibara, K.; Fukushima, A.; Nakabayashi, R.; Hanada, K.; Matsuda, F.; Sugawara, S.; Inoue, E.; Kuromori, T.; Ito, T.; Shinozaki, K.; Wangwattana, B.; Yamazaki, M.; Saito, K.; Plant J. 69, 154-167 (2012)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene ugt79b1, DNA and amino acid sequence determination and analysis, expression analysis and phylogenetic analysis, recombinant expression as His-tagged protein in Escherichia coli
Arabidopsis thaliana
recombinantly expressed as a His-tagged fusion protein
Arabidopsis thaliana
Engineering
Protein Variants
Commentary
Organism
additional information
construction of transposon insertion mutants of UGT79B1
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
UDP-alpha-D-xylose + cyanidin 3-O-beta-D-glucoside
Arabidopsis thaliana
-
UDP + cyanidin 3-O-beta-D-sambubioside
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Arabidopsis thaliana
Q9LVW3
-
-
Arabidopsis thaliana
Q9LVW3
gene ugt79b1
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant His-tagged enzyme from Escherichia coli
Arabidopsis thaliana
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
enzyme converts cyanidin 3-O-glucoside to cyanidin 3-O-xylosyl(1-2)glucoside. Enzyme recognizes 3-O-glucosylated anthocyanidins/flavonols and uridine diphosphate (UDP)-xylose
723428
Arabidopsis thaliana
?
-
-
-
?
additional information
UGT79B1 recognizes 3-O-glucosylated anthocyanidins/flavonols and UDP-xylose, but not 3,5-O-diglucosylated anthocyanidins, substrate specificity, overview. No activity with cyanidin 3-O-rhamnoside, cyanidin 3,5-O-diglucoside, cyanidin 3-O-6''-O-4-coumaroylglucoside-5-O-glucoside, cyanidin 3-O-6''-O-p-coumaroylglucoside, kaempferol 3-O-rhanoside, and kaempferol 3-O-glucoside-7-O-rhamnoside. The enzyme is absolutely specific for UDP-xylose as donar substrate, no activity with UDP-arabinose, UDP-galactose, UDP-glucose, UDP-glucuronic acid, and UDP-rhamnose
723428
Arabidopsis thaliana
?
-
-
-
?
UDP-alpha-D-xylose + cyanidin 3-O-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + cyanidin 3-O-beta-D-xylosyl-(1->2)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside
-
-
-
?
UDP-alpha-D-xylose + cyanidin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + cyanidin 3-O-beta-D-sambubioside
-
-
-
?
UDP-alpha-D-xylose + delphinidin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + delphinidin 3-O-beta-D-sambubioside
-
-
-
?
UDP-alpha-D-xylose + kaempferol 3-O-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + kaempferol 3-O-beta-D-xylosyl-(1->2)-[alpha-L-rhamnosyl-(1->6)]-beta-D-glucoside
-
-
-
?
UDP-alpha-D-xylose + kaempferol 3-O-beta-D-glucoside
best substrate
723428
Arabidopsis thaliana
UDP + kaempferol 3-O-beta-D-xylosyl-(1->2)-beta-D-glucoside
-
-
-
?
UDP-alpha-D-xylose + pelargonidin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + pelargonidin 3-O-beta-D-sambubioside
-
-
-
?
UDP-alpha-D-xylose + quercetin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + quercetin 3-O-beta-D-sambubioside
-
-
-
?
UDP-D-xylose + cyanidin 3-O-(alpha-L-rhamnosyl-(1->6)-beta-D-glucoside)
-
723428
Arabidopsis thaliana
UDP + cyanidin 3-O-(beta-D-xylosyl-1(->2)[alpha-L-rhamnosyl-(1->6)]-beta-D-glucoside)
-
-
-
?
UDP-D-xylose + cyanidin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + cyanidin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
-
-
-
?
UDP-D-xylose + delphinidin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + delphinidin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
-
-
-
?
UDP-D-xylose + kaempferol 3-O-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + kaempferol 3-O-(beta-D-xylosyl-(1->2)[alpha-L-rhamnosyl-(1->6)]-beta-D-glucoside)
-
-
-
?
UDP-D-xylose + kaempferol 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + kaempferol 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
-
-
-
?
UDP-D-xylose + pelargonidin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + pelargonidin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
-
-
-
?
UDP-D-xylose + quercetin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + quercetin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
anthocyanin 3-O-glucoside: 2''-O-xylosyltransferase
-
Arabidopsis thaliana
UGT79B1
-
Arabidopsis thaliana
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Arabidopsis thaliana
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Arabidopsis thaliana
7.5
-
assay at
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
gene ugt79b1, DNA and amino acid sequence determination and analysis, expression analysis and phylogenetic analysis, recombinant expression as His-tagged protein in Escherichia coli
Arabidopsis thaliana
recombinantly expressed as a His-tagged fusion protein
Arabidopsis thaliana
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
construction of transposon insertion mutants of UGT79B1
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
UDP-alpha-D-xylose + cyanidin 3-O-beta-D-glucoside
Arabidopsis thaliana
-
UDP + cyanidin 3-O-beta-D-sambubioside
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
enzyme converts cyanidin 3-O-glucoside to cyanidin 3-O-xylosyl(1-2)glucoside. Enzyme recognizes 3-O-glucosylated anthocyanidins/flavonols and uridine diphosphate (UDP)-xylose
723428
Arabidopsis thaliana
?
-
-
-
?
additional information
UGT79B1 recognizes 3-O-glucosylated anthocyanidins/flavonols and UDP-xylose, but not 3,5-O-diglucosylated anthocyanidins, substrate specificity, overview. No activity with cyanidin 3-O-rhamnoside, cyanidin 3,5-O-diglucoside, cyanidin 3-O-6''-O-4-coumaroylglucoside-5-O-glucoside, cyanidin 3-O-6''-O-p-coumaroylglucoside, kaempferol 3-O-rhanoside, and kaempferol 3-O-glucoside-7-O-rhamnoside. The enzyme is absolutely specific for UDP-xylose as donar substrate, no activity with UDP-arabinose, UDP-galactose, UDP-glucose, UDP-glucuronic acid, and UDP-rhamnose
723428
Arabidopsis thaliana
?
-
-
-
?
UDP-alpha-D-xylose + cyanidin 3-O-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + cyanidin 3-O-beta-D-xylosyl-(1->2)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside
-
-
-
?
UDP-alpha-D-xylose + cyanidin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + cyanidin 3-O-beta-D-sambubioside
-
-
-
?
UDP-alpha-D-xylose + delphinidin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + delphinidin 3-O-beta-D-sambubioside
-
-
-
?
UDP-alpha-D-xylose + kaempferol 3-O-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + kaempferol 3-O-beta-D-xylosyl-(1->2)-[alpha-L-rhamnosyl-(1->6)]-beta-D-glucoside
-
-
-
?
UDP-alpha-D-xylose + kaempferol 3-O-beta-D-glucoside
best substrate
723428
Arabidopsis thaliana
UDP + kaempferol 3-O-beta-D-xylosyl-(1->2)-beta-D-glucoside
-
-
-
?
UDP-alpha-D-xylose + pelargonidin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + pelargonidin 3-O-beta-D-sambubioside
-
-
-
?
UDP-alpha-D-xylose + quercetin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + quercetin 3-O-beta-D-sambubioside
-
-
-
?
UDP-D-xylose + cyanidin 3-O-(alpha-L-rhamnosyl-(1->6)-beta-D-glucoside)
-
723428
Arabidopsis thaliana
UDP + cyanidin 3-O-(beta-D-xylosyl-1(->2)[alpha-L-rhamnosyl-(1->6)]-beta-D-glucoside)
-
-
-
?
UDP-D-xylose + cyanidin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + cyanidin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
-
-
-
?
UDP-D-xylose + delphinidin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + delphinidin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
-
-
-
?
UDP-D-xylose + kaempferol 3-O-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + kaempferol 3-O-(beta-D-xylosyl-(1->2)[alpha-L-rhamnosyl-(1->6)]-beta-D-glucoside)
-
-
-
?
UDP-D-xylose + kaempferol 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + kaempferol 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
-
-
-
?
UDP-D-xylose + pelargonidin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + pelargonidin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
-
-
-
?
UDP-D-xylose + quercetin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + quercetin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Arabidopsis thaliana
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Arabidopsis thaliana
7.5
-
assay at
Arabidopsis thaliana
General Information
General Information
Commentary
Organism
evolution
flavonoid UGT phylogenetic tree, UGT79B1 belongs to a cluster in which UGTs transfer a glycosyl group to a sugar moiety of flavonoid glycosides
Arabidopsis thaliana
malfunction
anthocyanin is drastically reduced in ugt79b1 knockout mutants
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
evolution
flavonoid UGT phylogenetic tree, UGT79B1 belongs to a cluster in which UGTs transfer a glycosyl group to a sugar moiety of flavonoid glycosides
Arabidopsis thaliana
malfunction
anthocyanin is drastically reduced in ugt79b1 knockout mutants
Arabidopsis thaliana
Other publictions for EC 2.4.2.51
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723428
Yonekura-Sakakibara
Two glycosyltransferases invol ...
Arabidopsis thaliana
Plant J.
69
154-167
2012
-
-
2
-
1
-
-
-
-
-
-
1
-
4
-
-
1
-
-
-
-
-
16
-
2
1
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
16
-
1
-
-
-
2
-
-
-
-
2
2
-
-
-
720781
Teusch
Uridine 5'-diphosphate-xylose: ...
Matthiola incana
Planta
169
559-563
1986
-
-
-
-
-
-
13
-
-
1
-
5
-
2
-
-
-
-
-
3
-
-
5
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13
-
-
-
1
-
5
-
-
-
-
-
3
-
-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-