Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.2.47 extracted from

  • Tan, Y.; Zhang, L.; Rodrigues, J.; Zheng, R.; Giacometti, S.; Rosario, A.; Kloss, B.; Dandey, V.; Wei, H.; Brunton, R.; Raczkowski, A.; Athayde, D.; Catalao, M.; Pimentel, M.; Clarke, O.; Lowary, T.; Archer, M.; Niederweis, M.; Potter, C.; Carragher, B.
    Cryo-EM structures and regulation of arabinofuranosyltransferase AftD from Mycobacteria (2020), Mol. Cell., 78, 683-699.e11 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.2.47

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) pLysS cells and Mycobacterium smegmatis DELTAaftD mutants Mycobacteroides abscessus

Protein Variants

Protein Variants Comment Organism
D41A inactive Mycobacteroides abscessus
D474A inactive Mycobacteroides abscessus
E251A inactive Mycobacteroides abscessus
K361A inactive Mycobacteroides abscessus
K39A inactive Mycobacteroides abscessus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Mycobacteroides abscessus 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
arabinogalactan + decaprenyl phosphoryl-D-arabinose Mycobacteroides abscessus the enzyme performs alpha-(1->3) branching of arabinogalactan ?
-
 ?
lipoarabinomannan + decaprenyl phosphoryl-D-arabinose Mycobacteroides abscessus the enzyme performs alpha-(1->3) branching of lipoarabinomannan ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacteroides abscessus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA resin column chromatography Mycobacteroides abscessus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arabinogalactan + decaprenyl phosphoryl-D-arabinose the enzyme performs alpha-(1->3) branching of arabinogalactan Mycobacteroides abscessus ?
-
 ?
lipoarabinomannan + decaprenyl phosphoryl-D-arabinose the enzyme performs alpha-(1->3) branching of lipoarabinomannan Mycobacteroides abscessus ?
-
 ?
additional information the enzyme is non-covalently complexed with an acyl carrier protein which regulates enzyme function Mycobacteroides abscessus ?
-
-

Subunits

Subunits Comment Organism
? x * 150000, calculated from amino acid sequence Mycobacteroides abscessus

Synonyms

Synonyms Comment Organism
AftD
-
 Mycobacteroides abscessus
arabinofuranosyltransferase D
-
 Mycobacteroides abscessus

General Information

General Information Comment Organism
physiological function the enzyme is essential for growth Mycobacteroides abscessus