Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli and in Spodoptera frugiperda Sf21 cells, via baculovirus infection, produced an insoluble protein, not successful | Phaseolus vulgaris |
in vitro transcription and translation, posttranslational processing by bean endosperm extract | Phaseolus vulgaris |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of transgenic tobacco plants via Agrobacterium tumefaciens infection, functional expression under control of the CaMV35S promotor in seedlings and leaves, transgenic plants are more sensitive to auxin than wild-type plants, phenotypic alterations are leaf chlorosis, restriction of root elongation and eventual cessation of growth | Phaseolus vulgaris |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Phaseolus vulgaris | 5829 | - |
nucleus | - |
Phaseolus vulgaris | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
an inactive form of MW about 70 kDa is detected by immunostaining in vegetative tissue prior to posttranslational processing of the protein | Phaseolus vulgaris |
50000 | - |
- |
Phaseolus vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaseolus vulgaris | - |
cv. Great Northern | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endosperm | - |
Phaseolus vulgaris | - |
seed | - |
Phaseolus vulgaris | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-xylose + zeatin | - |
Phaseolus vulgaris | UDP + O-beta-D-xylosylzeatin | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, SDS-PAGE | Phaseolus vulgaris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | - |
assay at | Phaseolus vulgaris |