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Literature summary for 2.4.2.40 extracted from
- Isolation and partial purification of an enzyme catalyzing the formation of O-xylosylzeatin in phaseolus vulgaris embryos (1987), Proc. Natl. Acad. Sci. USA, 84, 3714-3717.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
trans-zeatin | - |
Phaseolus vulgaris |  |
| 0.003 | - |
UDP-xylose | - |
Phaseolus vulgaris | |
| 0.01 | - |
dihydrozeatin | - |
Phaseolus vulgaris | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Phaseolus vulgaris | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
gel filtration | Phaseolus vulgaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| no activity in Phaseolus lunatus | - |
- |
- |
| Phaseolus vulgaris | - |
cv. Great Northern | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Phaseolus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | immature | Phaseolus vulgaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0049 | - |
- |
Phaseolus vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-D-xylose + dihydrozeatin | - |
Phaseolus vulgaris | UDP + O-beta-D-xylosyldihydrozeatin | - |
? | |
| UDP-D-xylose + zeatin | absolutely specific for UDP-D-xylose | Phaseolus vulgaris | UDP + O-beta-D-xylosylzeatin | - |
? | |
| UDP-D-xylose + zeatin | trans-zeatin | Phaseolus vulgaris | UDP + O-beta-D-xylosylzeatin | - |
? | |
| UDP-D-xylose + zeatin | cis-zeatin and ribosylzeatin are no substrates | Phaseolus vulgaris | UDP + O-beta-D-xylosylzeatin | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Phaseolus vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | - |
Phaseolus vulgaris |
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Release 2023.1 (January 2023)
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