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Literature summary for 2.4.2.4 extracted from

  • Blank, J.G.; Hoffee, P.A.
    Purification and properties of thymidine phosphorylase from Salmonella typhimurium (1975), Arch. Biochem. Biophys., 168, 259-265.
    View publication on PubMed
Search for more literature for 2.4.2.4

General Stability

General Stability Organism
2-mercaptoethanol and sucrose stabilize during preincubation procedure with ammonium sulfate Salmonella enterica subsp. enterica serovar Typhimurium
quite labile during purification Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors Comment Organism Structure
2-deoxy-D-ribose product inhibition Salmonella enterica subsp. enterica serovar Typhimurium
5-Hydroxymethyluracil 35% inactivation at 2 mM Salmonella enterica subsp. enterica serovar Typhimurium
Bromouracil 100% inactivation at 1 mM Salmonella enterica subsp. enterica serovar Typhimurium
deoxyadenosine 20% inactivation at 1 mM Salmonella enterica subsp. enterica serovar Typhimurium
ribose 1-phosphate 20% inactivation at 1.5 mM Salmonella enterica subsp. enterica serovar Typhimurium
thymine product inhibition Salmonella enterica subsp. enterica serovar Typhimurium
Uracil 84% inactivation at 2 mM Salmonella enterica subsp. enterica serovar Typhimurium
uridine competitive 27% inactivation at 1 mM Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
 arsenate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.1
-
 thymidine
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.3
-
 phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
8
-
 deoxyuridine
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 2 * 47000, SDS-disc gel electrophoresis Salmonella enterica subsp. enterica serovar Typhimurium
100000
-
 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
 LT-2
-

Purification (Commentary)

Purification (Comment) Organism
257fold Salmonella enterica subsp. enterica serovar Typhimurium

Reaction

Reaction Comment Organism Reaction ID
thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate ordered bi bi mechanism with the nucleoside the first substrate to add, and the pyrimidine base the last product to leave Salmonella enterica subsp. enterica serovar Typhimurium
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
465
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

Storage Stability Organism
4°C, 10 mM potassium phosphate, pH 7.5, 10 mM 2-mercaptoethanol, 20% sucrose, stable for 3 months Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bromodeoxyuridine + phosphate at 40% the rate of thymidine Salmonella enterica subsp. enterica serovar Typhimurium bromouracil + 2-deoxy-D-ribose
-
 ?
deoxyuridine + phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium uracil + 2-deoxy-D-ribose 1-phosphate
-
 ?
iododeoxyuridine + phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium iodouracil + 2-deoxy-D-ribose
-
 ?
additional information no substrate: deoxycytidine Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
additional information no substrates: deoxyadenosine, deoxyguanosine Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
additional information specific for deoxyribonucleosides Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
thymidine + arsenate
-
 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
thymidine + phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium thymine + 2-deoxy-D-ribose 1-phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 47000, SDS-disc gel electrophoresis Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 rapid decrease of activity above and below this range Salmonella enterica subsp. enterica serovar Typhimurium