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Literature summary for 2.4.2.4 extracted from
- Thymidine phosphorylase from Salmonella typhimurium (1978), Methods Enzymol., 51, 437-442.
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol and sucrose stabilize during preincubation procedure with ammonium sulfate | Salmonella enterica subsp. enterica serovar Typhimurium |
| quite labile during purification | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.3 | - |
arsenate | - |
Salmonella enterica subsp. enterica serovar Typhimurium |  |
| 2.1 | - |
thymidine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| 2.3 | - |
phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| 8 | - |
deoxyuridine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
2 * 47000 | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thymine + 2-deoxy-D-ribose 1-phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | in Escherichia coli and Salmonella typhimurium thymidine phosphorylase plays an important role in metabolism of thymine auxotrophs and is necessary for the conversion of exogenous thymine to thymidine | thymidine + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 465 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 10 mM potassium phosphate, pH 7.5, 10 mM 2-mercaptoethanol, 20% sucrose, stable for 3 months | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bromodeoxyuridine + phosphate | at 40% the rate of thymidine | Salmonella enterica subsp. enterica serovar Typhimurium | bromouracil + 2-deoxy-D-ribose | - |
? | |
| deoxyuridine + phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | uracil + 2-deoxy-D-ribose 1-phosphate | - |
? | |
| iododeoxyuridine + phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | iodouracil + 2-deoxy-D-ribose | - |
? | |
| additional information | no substrate: deoxycytidine | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| additional information | no substrates: deoxyadenosine, deoxyguanosine | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| thymidine + arsenate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| thymidine + phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | thymine + 2-deoxy-D-ribose 1-phosphate | - |
? | |
| thymine + 2-deoxy-D-ribose 1-phosphate | in Escherichia coli and Salmonella typhimurium thymidine phosphorylase plays an important role in metabolism of thymine auxotrophs and is necessary for the conversion of exogenous thymine to thymidine | Salmonella enterica subsp. enterica serovar Typhimurium | thymidine + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 47000 | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
| 7.5 | 8 | rapid decrease of activity above and below this range | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | rapid decrease of activity above and below this range | Salmonella enterica subsp. enterica serovar Typhimurium |
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