Cloned (Comment) | Organism |
---|---|
in pENTR/D-TOPO downstream of cauliflower mosaic virus 35S promoter for recombination with pEarleyGate 201 and Agrobacterium tumefacies strain GV3101-mediated transformation (floral-flip), expression with hemagglutinin (HA) tag | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | immunolocalization of recombinant enzyme using tag-specific antibody, predicted Golgi-resident type-II membrane protein | Arabidopsis thaliana | 5794 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
predicted from primary structure | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-xylose + (glucosyl)xyloglucan | Arabidopsis thaliana | - |
UDP + (xylosyl-glucosyl)xyloglucan | reduction of xyloglucan content (reduced glucan backbone substitution with xylose) in plants that lack functional XXT5 can be complemented by transformation with recombinant XXT5 | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9CA75 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cauline leaf | high expression level, revealed by Reverse Transcription-PCR | Arabidopsis thaliana | - |
plant | expressed in all major organs, revealed by Reverse Transcription-PCR | Arabidopsis thaliana | - |
root | high expression level, revealed by Reverse Transcription-PCR, shorter root hair and less root tissue xyloglucan detectable upon lack of XXT5 | Arabidopsis thaliana | - |
seed vessel | silique, lowest expression level, revealed by Reverse Transcription-PCR | Arabidopsis thaliana | - |
stem | high expression level, revealed by Reverse Transcription-PCR | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no xylosyltransferase activity in vitro after expression in either Pichia pastoris or Sf21 insect cells using UDP-xylose and a broad range of putative acceptors | Arabidopsis thaliana | ? | - |
? | |
UDP-D-xylose + (glucosyl)xyloglucan | - |
Arabidopsis thaliana | UDP + (xylosyl-glucosyl)xyloglucan | reduction of xyloglucan content (reduced glucan backbone substitution with xylose) in plants that lack functional XXT5 can be complemented by transformation with recombinant XXT5 | ? |
Synonyms | Comment | Organism |
---|---|---|
At1g74380 | - |
Arabidopsis thaliana |
XXT5 | putative xyloglycan xylosylransferase | Arabidopsis thaliana |