Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression of full length wild-type enzyme and diverse mutants in Spodoptera frugiperda Sf9 insect cells via baculovirus infection system as soluble proteins lacking the transmembrane domain, expression of the full length enzyme and enzyme fragments as GFP-fusion proteins in tobacco cells Golgi medial cisternae via Agrobacterium tumefaciens infection system for study of enzyme subcellular localization | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | serial deletions at both the N- and the C-terminal ends, integrity of a large domain located between amino acid 31 and the C-terminal lumenal region is required for activity, construction of transgenic tobacco expressing the enzyme via Agrobacterium tumefaciens infection system | Arabidopsis thaliana |
S53A | site-directed mutagenesis, possesses the N-glycosylation sites Asn301 and Asn478 | Arabidopsis thaliana |
S53A/T303A | site-directed mutagenesis | Arabidopsis thaliana |
T303A | site-directed mutagenesis, possesses the N-glycosylation sites Asn51 and Asn478 | Arabidopsis thaliana |
T480A | site-directed mutagenesis, possesses the N-glycosylation sites Asn51 and Asn301 | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | strong inhibition | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | medial cisternae | Arabidopsis thaliana | 5794 | - |
membrane | enzyme possesses a transmembrane domain | Arabidopsis thaliana | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | highly required for activity | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-xylose + N4-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl]asparagine | Arabidopsis thaliana | - |
UDP + N4-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-[beta-D-xylosyl-(1,2)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl]asparagine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9LDH0 | - |
- |
no activity in Spodoptera frugiperda | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 3 potential N-glycosylation sites, Asn51, Asn301, and Asn478, 2 of which are glycosylated, i.e. Asn51 and Asn301, occupancy of at least one site is necessary for enzyme activity and stability, glycosylation sites of diverse mutants, overview | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity of truncated enzyme mutants | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity, substrates need to possess a beta(1,2)-linked GlcNAc residue at the Man-alpha-(1,3) arm, Man-alpha-(1,6)-(Man-alpha(1,3))-Man-beta(1-R) is no acceptor substrate, integrity of a large domain located between amino acid 31 and the C-terminal lumenal region is required for activity, the cytosolic tail and the transmembrane region are not essential for activity, but the stem region and the C-terminal end are | Arabidopsis thaliana | ? | - |
? | |
UDP-D-xylose + alpha-D-mannosyl-(1,6)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)]-beta-D-mannosyl-O-(CH2)7-CH3 | - |
Arabidopsis thaliana | UDP + ? | - |
? | |
UDP-D-xylose + N4-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl]asparagine | - |
Arabidopsis thaliana | UDP + N4-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-[beta-D-xylosyl-(1,2)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl]asparagine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta1,2-xylosyltransferase | - |
Arabidopsis thaliana |
XylT | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | negligible activity at pH 5.0 | Arabidopsis thaliana |