Literature summary for 2.4.2.31 extracted from

Tsurumura, T.; Tsumori, Y.; Qiu, H.; Oda, M.; Sakurai, J.; Nagahama, M.; Tsuge, H.
Arginine ADP-ribosylation mechanism based on structural snapshots of iota-toxin and actin complex (2013), Proc. Natl. Acad. Sci. USA, 110, 4267-4272.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Clostridium perfringens

Crystallization (Commentary)

Crystallization (Comment)	Organism
high-resolution structures of NAD(+)-iota toxin-actin and iota toxin-ADPR-actin obtained by soaking apo-iota toxin-actin crystal with NAD(+) under different conditions and structures of mutants E378S, E380A, E380S in complex with actin. The structures of NAD(+)-iota toxin-actin and iota toxin-ADPR-actin represent the pre- and postreaction states. A simple strain-alleviation model explains arginine ADP ribosylation occuring via two oxocarbenium ion intermediates	Clostridium perfringens

Crystallization (Comment)

Organism

high-resolution structures of NAD(+)-iota toxin-actin and iota toxin-ADPR-actin obtained by soaking apo-iota toxin-actin crystal with NAD(+) under different conditions and structures of mutants E378S, E380A, E380S in complex with actin. The structures of NAD(+)-iota toxin-actin and iota toxin-ADPR-actin represent the pre- and postreaction states. A simple strain-alleviation model explains arginine ADP ribosylation occuring via two oxocarbenium ion intermediates

Clostridium perfringens

Protein Variants

Protein Variants	Comment	Organism
E378S	mutation eliminates ADP-ribosylation activity and reduces the weak NAD+ glycohydrolase activity in absence of actin to 50%	Clostridium perfringens
E380A	mutation eliminates both ADP-ribosylation activity and the weak NAD+ glycohydrolase activity in absence of actin	Clostridium perfringens
E380S	mutation eliminates both ADP-ribosylation activity and the weak NAD+ glycohydrolase activity in absence of actin	Clostridium perfringens

Organism

Organism	UniProt	Comment	Textmining
Clostridium perfringens	Q46220	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	in absence of actin, enzyme displays weak NAD+ glycohydrolase activity	Clostridium perfringens	?	-	?
NAD+ + [actin]-L-arginine177	-	Clostridium perfringens	nicotinamide + [actin]-N-(ADP-D-ribosyl)-L-arginine177 + H+	-	?