Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Clostridium perfringens |
Crystallization (Comment) | Organism |
---|---|
high-resolution structures of NAD(+)-iota toxin-actin and iota toxin-ADPR-actin obtained by soaking apo-iota toxin-actin crystal with NAD(+) under different conditions and structures of mutants E378S, E380A, E380S in complex with actin. The structures of NAD(+)-iota toxin-actin and iota toxin-ADPR-actin represent the pre- and postreaction states. A simple strain-alleviation model explains arginine ADP ribosylation occuring via two oxocarbenium ion intermediates | Clostridium perfringens |
Protein Variants | Comment | Organism |
---|---|---|
E378S | mutation eliminates ADP-ribosylation activity and reduces the weak NAD+ glycohydrolase activity in absence of actin to 50% | Clostridium perfringens |
E380A | mutation eliminates both ADP-ribosylation activity and the weak NAD+ glycohydrolase activity in absence of actin | Clostridium perfringens |
E380S | mutation eliminates both ADP-ribosylation activity and the weak NAD+ glycohydrolase activity in absence of actin | Clostridium perfringens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium perfringens | Q46220 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | in absence of actin, enzyme displays weak NAD+ glycohydrolase activity | Clostridium perfringens | ? | - |
? | |
NAD+ + [actin]-L-arginine177 | - |
Clostridium perfringens | nicotinamide + [actin]-N-(ADP-D-ribosyl)-L-arginine177 + H+ | - |
? |