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Literature summary for 2.4.2.31 extracted from

  • Grahnert, A.; Richter, S.; Siegert, F.; Berndt, A.; Hauschildt, S.
    The orthologue of the "acatalytic" mammalian ART4 in chicken is an arginine-specific mono-ADP-ribosyltransferase (2008), BMC Mol. Biol., 9, 86.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.2.31

Cloned(Commentary)

Cloned (Comment) Organism
N-terminal Flag-tagged ART4 (amino acids 20-270) cloned (Asp718I/XhoI) into the pSecTagB plasmid. HEK-293T cells stably transfected with the plasmid. C-33A cells stably transfected with an expression plasmid containing the sequence of ART4 encoding amino acids 20-289 Gallus gallus
wild-type and mutants cloned into the pcDNA3.1/Zeo (+) plasmid, expression in C-33A cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
K242E displays no activity Homo sapiens
Y187R displays no activity Homo sapiens
Y187R/K242E double mutant contains the R-S-EXE motif, but displays no activity, thus additional residues besides the intact R-S-EXE motif are involved in catalyzing the enzymatic reaction Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0007
-
 NAD+ recombinant ART4, in the presence of PNGase F Gallus gallus
0.001
-
 NAD+ recombinant ART4 Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information upon ectopic expression in C-33A cells, recombinant chicken ART4 localizes at the cell surface as a glycosyl-phosphatidylinositol-anchored, highly glycosylated protein, which displays arginine-specific ART activity Gallus gallus
-
-

Organism

Organism UniProt Comment Textmining
Gallus gallus B6RDZ5
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by affinity chromatography Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
bone marrow high level Gallus gallus
-
bursa of Fabricius very low level Gallus gallus
-
liver high level Gallus gallus
-
additional information very low level in caecum, absent from lung Gallus gallus
-
spleen high level Gallus gallus
-
thymus very low level Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with NAD+ as substrate Homo sapiens ?
-
 ?
polyarginine + NAD+ in contrast to its mammalian orthologues, the chicken protein contains an intact R-S-EXE motif Gallus gallus nicotinamide + ?
-
 ?

Synonyms

Synonyms Comment Organism
arginine-specific mono-ADP-ribosyltransferase
-
 Homo sapiens
arginine-specific mono-ADP-ribosyltransferase
-
 Gallus gallus
ART4
-
 Homo sapiens
ART4
-
 Gallus gallus

General Information

General Information Comment Organism
physiological function avian orthologue of the acatalytic mammalian ART4 is a mono-ADP-ribosyltransferase with enzymatic activity comparable to that of other, catalytically active and glycosyl-phosphatidylinositol-anchored members of the mammalian ART family Gallus gallus
physiological function in contrast to its avian orthologue the mammalian ART4 is a acatalytic mono-ADP-ribosyltransferase Homo sapiens