Cloned (Comment) | Organism |
---|---|
expression of GST-tagged wild-type and mutant enzymes in Escherichia coli | Hathewaya limosa |
Protein Variants | Comment | Organism |
---|---|---|
E219Q | site-directed mutagenesis, almost inactive mutant | Hathewaya limosa |
Q217E | site-directed mutagenesis, the mutation results in inhibition of the enzyme's ADP-ribosyltransferase activity toward RhoA, the mutant protein is still capable of NAD+-binding and possesses NAD+ glycohydrolase activity, the mutant is capable of ADP-ribosylation of poly-arginine but not poly-asparagine | Hathewaya limosa |
R151A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Hathewaya limosa |
R61A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Hathewaya limosa |
R86A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Hathewaya limosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Hathewaya limosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hathewaya limosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli by glutathione affinity chromatography, the GST-tag is cleaved off by thrombin | Hathewaya limosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme exhibits ADP-ribosyltransferase and NAD+ glycohydrolase activity, modeling of interaction od substrate and catalytic residues, overview | Hathewaya limosa | ? | - |
? | |
RhoA + NAD+ | the enzyme modifies the low-molecular-mass GTPase RhoA specifically at Asn41 | Hathewaya limosa | Nomega-(ADP-D-ribosyl)-L-asparagine41-RhoA + nicotinamide | - |
? | |
RhoB + NAD+ | the enzyme modifies the low-molecular-mass GTPase RhoB specifically at Asn41 | Hathewaya limosa | Nomega-(ADP-D-ribosyl)-L-asparagine41-RhoB + nicotinamide | - |
? | |
RhoC + NAD+ | the enzyme modifies the low-molecular-mass GTPase RhoC specifically at Asn41 | Hathewaya limosa | Nomega-(ADP-D-ribosyl)-L-asparagine41-RhoC + nicotinamide | - |
? | |
soybean trypsin inhibitor + NAD+ | - |
Hathewaya limosa | Nomega-(ADP-D-ribosyl)-L-arginine-soybean trypsin inhibitor + nicotinamide | - |
? | |
wild-type exoenzyme C3 + NAD+ | the recombinant mutant Q217E enzyme modifies the recombinant wild-type enzyme at Arg86 | Hathewaya limosa | Nomega-(ADP-D-ribosyl)-L-arginine86-wild-type exoenzyme C3 + nicotinamide | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme contains an ADP-ribosylating turn-turn motif, i.e. ARTT-motif, with the key residue Q217, that is involved in substrate specificity and recognition | Hathewaya limosa |
Synonyms | Comment | Organism |
---|---|---|
asparagine-specific ADP-ribosyltransferase | - |
Hathewaya limosa |
exoenzyme C3 | - |
Hathewaya limosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 37 | assay at | Hathewaya limosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Hathewaya limosa |