Crystallization (Comment) | Organism |
---|---|
structure of the Ia complex with NADH at 1.8 A. Vapor diffusion method | Clostridium perfringens |
Protein Variants | Comment | Organism |
---|---|---|
E301A | no NADase activity detected | Clostridium perfringens |
F349A | the ratio of turnover-number to Km-value in the NADase reaction is 1% of the wild-type ratio. The ratio of turnover-number to Km-value in the ADP-ribosyltransferase activity is 1.9% of the wild-type ratio with rabbit muscle actin as substrate and 4% of the wild-type ratio with non-muscle actin from human platelets as substrate | Clostridium perfringens |
F349Y | the ratio of turnover-number to Km-value in the NADase reaction is 110% of the wild-type ratio | Clostridium perfringens |
N255A | the ratio of turnover-number to Km-value in the NADase reaction is 90% of the wild-type ratio. The ratio of turnover-number to Km-value in the ADP-ribosyltransferase activity is 20% of the wild-type ratio with rabbit muscle actin as substrate and 77% of the wild-type ratio with non-muscle actin from human platelets as substrate | Clostridium perfringens |
R352A | no ADP-ribosyltransferase activity with rabbit muscle actin and non-muscle actin from human platelets | Clostridium perfringens |
R352A | the ratio of turnover-number to Km-value in the NADase reaction is 2% of the wild-type ratio, no activity | Clostridium perfringens |
Y246A | the ratio of turnover-number to Km-value in the NADase reaction is 90% of the wild-type ratio. The ratio of turnover-number to Km-value in the ADP-ribosyltransferase activity is 1.9% of the wild-type ratio with rabbit muscle actin as substrate and 19% of the wild-type ratio with non-muscle actin from human platelets as substrate | Clostridium perfringens |
Y251A | the ratio of turnover-number to Km-value in the NADase reaction is 10% of the wild-type ratio | Clostridium perfringens |
Y251F | the ratio of turnover-number to Km-value in the NADase reaction is 40% of the wild-type ratio | Clostridium perfringens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
NAD+ | ADP-ribosyltransferase activity, mutant enzyme F349A, cosubstrate: non-muscle actin from human platelets | Clostridium perfringens | |
0.58 | - |
NAD+ | ADP-ribosyltransferase activity, mutant enzyme N255A, cosubstrate: non-muscle actin from human platelets | Clostridium perfringens | |
0.69 | - |
NAD+ | ADP-ribosyltransferase activity, mutant enzyme F349A, cosubstrate: rabbit muscle actin | Clostridium perfringens | |
0.77 | - |
NAD+ | ADP-ribosyltransferase activity, wild-type enzyme, cosubstrate: non-muscle actin from human platelets | Clostridium perfringens | |
0.92 | - |
NAD+ | ADP-ribosyltransferase activity, mutant enzyme Y246A, cosubstrate: non-muscle actin from human platelets | Clostridium perfringens | |
0.93 | - |
NAD+ | ADP-ribosyltransferase activity, mutant enzyme Y246A, cosubstrate: rabbit muscle actin | Clostridium perfringens | |
1.28 | - |
NAD+ | ADP-ribosyltransferase activity, wild-type enzyme, cosubstrate: rabbit muscle actin | Clostridium perfringens | |
4.48 | - |
NAD+ | ADP-ribosyltransferase activity, mutant enzyme N255A, cosubstrate: rabbit muscle actin | Clostridium perfringens | |
8.67 | - |
NAD+ | ADP-ribosyltransferase activity, mutant enzyme Y251A, cosubstrate: non-muscle actin from human platelets | Clostridium perfringens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium perfringens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + non-muscle actin from human platelets | - |
Clostridium perfringens | nicotinamide + (ADP-D-ribosyl)-non-muscle actin from human platelets | - |
? | |
NAD+ + rabbit muscle actin | - |
Clostridium perfringens | nicotinamide + (ADP-D-ribosyl)-rabbit muscle actin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ARTase | - |
Clostridium perfringens |
i-toxin | - |
Clostridium perfringens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000067 | - |
NAD+ | ADP-ribosyltransferase activity, mutant enzyme F349A, cosubstrate: non-muscle actin from human platelets | Clostridium perfringens | |
0.00012 | - |
NAD+ | ADP-ribosyltransferase activity, mutant enzyme F349A, cosubstrate: rabbit muscle actin | Clostridium perfringens | |
0.00015 | - |
NAD+ | ADP-ribosyltransferase activity, mutant enzyme Y246A, cosubstrate: rabbit muscle actin | Clostridium perfringens | |
0.00133 | - |
NAD+ | ADP-ribosyltransferase activity, mutant enzyme Y246A, cosubstrate: non-muscle actin from human platelets | Clostridium perfringens | |
0.00133 | - |
NAD+ | ADP-ribosyltransferase activity, mutant enzyme Y251A, cosubstrate: non-muscle actin from human platelets | Clostridium perfringens | |
0.00183 | - |
NAD+ | ADP-ribosyltransferase activity, mutant enzyme N255A, cosubstrate: non-muscle actin from human platelets | Clostridium perfringens | |
0.0062 | - |
NAD+ | ADP-ribosyltransferase activity, wild-type enzyme, cosubstrate: non-muscle actin from human platelets | Clostridium perfringens | |
0.01 | - |
NAD+ | ADP-ribosyltransferase activity, wild-type enzyme, cosubstrate: rabbit muscle actin | Clostridium perfringens | |
0.014 | - |
NAD+ | ADP-ribosyltransferase activity, mutant enzyme N255A, cosubstrate: rabbit muscle actin | Clostridium perfringens |