Activating Compound | Comment | Organism | Structure |
---|---|---|---|
protein 14-3-3 | required, interaction analysis, interaction equires residues L426, D427, and L428, overview, binding study of mutant enzymes, overview | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
expression of various GST-fusion protein constructs of ExoS mutants in HeLa cells | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
A425K | site-directed mutagenesis, phenotypic analysis | Pseudomonas aeruginosa |
D424A | site-directed mutagenesis, phenotypic analysis | Pseudomonas aeruginosa |
D424A/D427A | site-directed mutagenesis, reduced interaction with 14-3-3, phenotypic analysis | Pseudomonas aeruginosa |
D424A/L426A/D427A/L428A | site-directed mutagenesis, the mutant shows a highly reduced expression level, phenotypic analysis | Pseudomonas aeruginosa |
D427A | site-directed mutagenesis, the mutant shows a reduced expression level, phenotypic analysis | Pseudomonas aeruginosa |
G421A | site-directed mutagenesis, phenotypic analysis | Pseudomonas aeruginosa |
L422A | site-directed mutagenesis, phenotypic analysis | Pseudomonas aeruginosa |
L423A | site-directed mutagenesis, phenotypic analysis | Pseudomonas aeruginosa |
L426A | site-directed mutagenesis, phenotypic analysis | Pseudomonas aeruginosa |
L426A/D427A | site-directed mutagenesis, the mutant shows a highly reduced expression level and slightly reduced cytotoxicity, no interaction with 14-3-3, phenotypic analysis | Pseudomonas aeruginosa |
L426A/D427A/L428A | site-directed mutagenesis, the mutant shows a highly reduced expression level and cytotoxicity, no interaction with 14-3-3, phenotypic analysis | Pseudomonas aeruginosa |
L428A | site-directed mutagenesis, reduced interaction with 14-3-3, the mutant shows highly reduced cytotoxicity, phenotypic analysis | Pseudomonas aeruginosa |
Q420A | site-directed mutagenesis, phenotypic analysis | Pseudomonas aeruginosa |
S419I | site-directed mutagenesis, phenotypic analysis | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas aeruginosa | the bacterial enzyme shows the ability to mediate cell death in the host and is a toxin, residues 426-428 are required for this activity | ? | - |
? | |
NAD+ + (ADP-D-ribosyl)n-acceptor | Pseudomonas aeruginosa | - |
nicotinamide + (ADP-D-ribosyl)n+1-acceptor | - |
? | |
NAD+ + Ras | Pseudomonas aeruginosa | interaction requires residue Leu428 | nicotinamide + (ADP-D-ribosyl)-Ras | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
gene exoS | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
NAD+ + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H+ | L428 is essential for activity | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the bacterial enzyme shows the ability to mediate cell death in the host and is a toxin, residues 426-428 are required for this activity | Pseudomonas aeruginosa | ? | - |
? | |
NAD+ + (ADP-D-ribosyl)n-acceptor | - |
Pseudomonas aeruginosa | nicotinamide + (ADP-D-ribosyl)n+1-acceptor | - |
? | |
NAD+ + Ras | interaction requires residue Leu428 | Pseudomonas aeruginosa | nicotinamide + (ADP-D-ribosyl)-Ras | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADP-ribosyltransferase | - |
Pseudomonas aeruginosa |
exoenzyme S | - |
Pseudomonas aeruginosa |
ExoS | - |
Pseudomonas aeruginosa |