Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DNA | absolute requirement | Dictyostelium discoideum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-dihydro-5-hydroxy-1,4-phthalazinedione | 0.001 mM, 95% inhibition of the 116000 Da enzyme | Dictyostelium discoideum | |
3-aminobenzamide | - |
Dictyostelium discoideum | |
3-Aminophthalhydrazide | 0.1 mM, 98% inhibition of the 116000 Da enzyme | Dictyostelium discoideum | |
3-Guanidinobenzamide | 0.1 mM, 71% inhibition of the 116000 Da enzyme | Dictyostelium discoideum | |
3-Hydroxybenzamide | 0.1 mM, 89% inhibition of the 116000 Da enzyme | Dictyostelium discoideum | |
3-Methoxybenzamide | 0.01 mM, 85% inhibition of the 116000 Da enzyme, 84% inhibition of the 90000 Da enzyme; 1 mM, 96% inhibition of the 116000 Da enzyme, 95% inhibition of the 90000 Da enzyme | Dictyostelium discoideum | |
Alpha-NAD+ | 0.5 mM, 40% inhibition of the 116000 Da enzyme, 44% inhibition of the 90000 Da enzyme | Dictyostelium discoideum | |
Caffeine | 1 mM, 86% inhibition of the 116000 Da enzyme, 57% inhibition of the 90000 Da enzyme | Dictyostelium discoideum | |
GTP(gammaS) | in presence of Mg2+ | Dictyostelium discoideum | |
KCl | 100 mM, 85% inhibition of the 90000 Da enzyme | Dictyostelium discoideum | |
nicotinamide | 1 mM, 96% inhibition of the 116000 Da enzyme, 95% inhibition of the 90000 Da enzyme | Dictyostelium discoideum | |
theophylline | 1 mM, 68% inhibition of the 116000 Da enzyme, 39% inhibition of the 90000 Da enzyme | Dictyostelium discoideum | |
thymidine | 1 mM, 94% inhibition of the 116000 Da enzyme, 88% inhibition of the 90000 Da enzyme | Dictyostelium discoideum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NAD+ | 90000 Da protein | Dictyostelium discoideum | |
0.077 | - |
NAD+ | 116000 Da protein | Dictyostelium discoideum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
1 * 90000, two active proteins are detected: MW 90000 Da and 116000 Da, SDS-PAGE | Dictyostelium discoideum |
116000 | - |
1 * 116000, two active proteins are detected: MW 90000 Da and 116000 Da, SDS-PAGE | Dictyostelium discoideum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium discoideum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Dictyostelium discoideum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0017 | - |
- |
Dictyostelium discoideum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (ADP-D-ribosyl)n-acceptor | poly(ADP-ribosyl)ation of histone H1 | Dictyostelium discoideum | nicotinamide + (ADP-D-ribosyl)n+1-acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 1 * 90000, two active proteins are detected: MW 90000 Da and 116000 Da, SDS-PAGE | Dictyostelium discoideum |
? | 1 * 116000, two active proteins are detected: MW 90000 Da and 116000 Da, SDS-PAGE | Dictyostelium discoideum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | - |
116000 Da enzyme | Dictyostelium discoideum |
10 | - |
90000 Da enzyme | Dictyostelium discoideum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
in presence of DNA and histone H1 | Dictyostelium discoideum |