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Literature summary for 2.4.2.3 extracted from
- Uridine phosphorylase from rat liver (1978), Methods Enzymol., 51, 423-431.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Rattus norvegicus |  |
| Deoxyglucosylthymine | phosphorolysis of uridine and deoxyuridine, synthesis of uridine at concentrations of 0.10 mM, 0.018 mM and 0.14 mM, not: phosphorolysis of deoxyuridine or thymidine at 0.19 mM | Rattus norvegicus | |
| iodoacetamide | - |
Rattus norvegicus | |
| iodoacetic acid | - |
Rattus norvegicus | |
| N-ethylmaleimide | - |
Rattus norvegicus | |
| o-Iodosobenzoate | - |
Rattus norvegicus | |
| p-chloromercuribenzoate | - |
Rattus norvegicus | |
| p-Mercuriphenylsulfonate | - |
Rattus norvegicus | |
| Uracil | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
4 * 26000, SDS-PAGE in presence of 4 M urea and 0.5% 2-mercaptoethanol | Rattus norvegicus |
| 110000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uridine + phosphate | Rattus norvegicus | role in degradation of pyrimidine nucleosides as well as in the salvage pathway for nucleic acid synthesis | uracil + alpha-D-ribose 1-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| uridine + phosphate = uracil + alpha-D-ribose 1-phosphate | ordered bi-bi mechanism | Rattus norvegicus | |
| uridine + phosphate = uracil + alpha-D-ribose 1-phosphate | phosphate binds before uridine and ribose 1-phosphate is released after uracil | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.4 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -40°C, 0.05 M potassium phosphate buffer, pH 7.0, 10 mM 2-mercaptoethanol, 1 mM EDTA, 10% loss of activity after 6 weeks | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uridine + phosphate | - |
Rattus norvegicus | uracil + alpha-D-ribose 1-phosphate | - |
r | |
| uridine + phosphate | role in degradation of pyrimidine nucleosides as well as in the salvage pathway for nucleic acid synthesis | Rattus norvegicus | uracil + alpha-D-ribose 1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 26000, SDS-PAGE in presence of 4 M urea and 0.5% 2-mercaptoethanol | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
reverse reaction, assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
deoxyuridine phosphorolysis | Rattus norvegicus |
| 8.2 | - |
reverse assay, uridine phosphorolysis | Rattus norvegicus |
| 8.5 | - |
uridine synthesis | Rattus norvegicus |
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