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Literature summary for 2.4.2.3 extracted from
- Expression, characterization, and detection of human uridine phosphorylase and identification of variant uridine phosphorolytic activity in selected human tumors (1998), Cancer Res., 58, 5418-5424.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| fusion protein with maltose-binding protein | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-benzylacyclouridine | 0.01 mM, 90% inhibition of normal tissues, 40-60% inhibition of carcinoma tissue | Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.076 | - |
ribose 1-phosphate | - |
Homo sapiens | |
| 0.189 | - |
uridine | - |
Homo sapiens | |
| 0.202 | - |
phosphate | - |
Homo sapiens | |
| 0.209 | - |
Uracil | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | 35000 | SDS-PAGE | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mus musculus |
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| colorectal adenocarcinoma cell | colon 26 tumor cells transplanted in BALB/c mice | Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 89.6 | - |
- |
Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxyuridine + phosphate | - |
Mus musculus | uracil + deoxyribose 1-phosphate | - |
? | |
| 2'-deoxyuridine + phosphate | - |
Homo sapiens | uracil + deoxyribose 1-phosphate | - |
? | |
| thymidine + phosphate | - |
Mus musculus | thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
| thymidine + phosphate | - |
Homo sapiens | thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
| uridine + phosphate | - |
Mus musculus | uracil + alpha-D-ribose 1-phosphate | - |
r | |
| uridine + phosphate | - |
Homo sapiens | uracil + alpha-D-ribose 1-phosphate | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.75 | pH 6.5: about 70% of activity maximum, pH 8.5: about 25% of activity maximum | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0073 | - |
5-benzylacyclouridine | - |
Homo sapiens | |
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