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Literature summary for 2.4.2.3 extracted from

  • Utagawa, T.; Morisawa, H.; Yamanaka, S.; Yamazaki, A.; Yoshinaga, F.; Hirose, Y.
    Properties of nucleoside phosphorylase from Enterobacter aerogenes (1985), Agric. Biol. Chem., 49, 3239-3246.
No PubMed abstract available
Search for more literature for 2.4.2.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
 uridine at 40°C Klebsiella aerogenes
1.8
-
 uridine at 60°C Klebsiella aerogenes

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
 AJ 11125
-
Klebsiella aerogenes AJ 11125
-
 AJ 11125
-

Purification (Commentary)

Purification (Comment) Organism
partial Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
97.83
-
-
 Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxyuridine + phosphate 18% of the activity with uridine Klebsiella aerogenes uracil + deoxyribose 1-phosphate
-
 ?
additional information no substrate: cytidine Klebsiella aerogenes ?
-
 ?
thymidine + phosphate 22% of the activity with uridine Klebsiella aerogenes thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
uracil arabinoside + phosphate 10% of the activity with uridine Klebsiella aerogenes uracil + arabinose-1-phosphate
-
 ?
uridine + phosphate
-
 Klebsiella aerogenes uracil + alpha-D-ribose 1-phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Klebsiella aerogenes

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80 40°C: about 40% of activity maximum, 80°C: about 10% of activity maximum Klebsiella aerogenes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 1 month, 20% loss of activity Klebsiella aerogenes
60
-
 half-life: around 1 week Klebsiella aerogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Klebsiella aerogenes

pH Range

pH Minimum pH Maximum Comment Organism
6.5 10.5 pH 6.5: about 50% of activity maximum, pH 10.5: about 65% of activity maximum Klebsiella aerogenes