Literature summary for 2.4.2.29 extracted from

Kittendorf, J.D.; Barcomb, L.M.; Nonekowski, S.T.; Garcia, G.A.
tRNA-guanine transglycosylase from Escherichia coli: Molecular mechanism and role of aspartate 89 (2001), Biochemistry, 40, 14123-14133.

Search for more literature for 2.4.2.29

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of D89E, D89D, D89C and D89A in Escherichia coli	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
D89A	less than 1% of the activity of the histidine-tagged wild-type enzyme	Escherichia coli
D89C	less than 1% of the activity of the histidine-tagged wild-type enzyme	Escherichia coli
D89E	about 50% of the activity of the histidine-tagged wild-type enzyme	Escherichia coli
D89N	less than 1% of the activity of the histidine-tagged wild-type enzyme	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0001	-	guanine	histidine-tagged wild-type enzyme, pH 7.3	Escherichia coli
0.00011	-	tRNA	histidine-tagged mutant enzyme D89E, pH 7.3	Escherichia coli
0.00012	-	tRNA	histidine-tagged wild-type enzyme, pH 7.3	Escherichia coli
0.00039	-	tRNA	histidine-tagged wild-type enzyme, pH 8.5	Escherichia coli
0.00051	-	tRNA	histidine-tagged mutant enzyme D89E, pH 8.5	Escherichia coli
0.00057	-	guanine	histidine-tagged wild-type enzyme, pH 8.5	Escherichia coli
0.00261	-	guanine	histidine-tagged mutant enzyme D89E, pH 7.3	Escherichia coli
0.00632	-	guanine	histidine-tagged mutant enzyme D89E, pH 8.5	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
wild-type and mutant enzymes D89E, D89D, D89C and D89A	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.59	-	guanine	histidine-tagged mutant enzyme D89E, pH 7.3	Escherichia coli
0.71	-	tRNA	histidine-tagged mutant enzyme D89E, pH 7.3	Escherichia coli
0.75	-	guanine	histidine-tagged mutant enzyme D89E, pH 8.5	Escherichia coli
0.93	-	tRNA	histidine-tagged mutant enzyme D89E, pH 8.5	Escherichia coli
1.21	-	guanine	histidine-tagged wild-type enzyme, pH 7.3	Escherichia coli
1.21	-	tRNA	histidine-tagged wild-type enzyme, pH 7.3	Escherichia coli
5.85	-	tRNA	histidine-tagged wild-type enzyme, pH 8.5	Escherichia coli
7.57	-	guanine	histidine-tagged wild-type enzyme, pH 8.5	Escherichia coli

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Release 2023.1 (January 2023)