Cloned (Comment) | Organism |
---|---|
different regions of MTAN cloned into the pGBT9.BS vector and transformed into Y190 yeast cells carrying pGAD.GH or pGAD.CBL3. Expressed in Escherichia coli BL21 cells carrying a GST fusion construct. MTAN promoter-GUS construct (pBI.tMTAN) transformed into Agrobacterium tumefaciens strain GV3101 and introduced into Arabidopsis plants by the floral dip method. 35S::CFP-CBL3-Myc and 35S::YFP-MTANHA constructs introduced, either alone or in combination, into Nicotiana benthamiana leaves by Agrobacterium tumefaciens-mediated infiltration method. CBL3-GFP and MTAN-GFP chimeric genes under the control of the cauliflower mosaic virus 35S promoter (pMD.CBL3 and pMD.MTAN) transiently expressed in Allium cepa epidermal cells. Onion epidermal cells bombarded with fusion constructs CBL3-YN and MTAN-YC | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
calcineurin B-like 3 | inhibits only in the presence of Ca2+, MTAN interacts specifically with calcineurin B-like 3, but not with calcineurin B-like 1 and calcineurin B-like 4. The 91-amino acid region of calcineurin B-like 3 is sufficient for the interaction with MTAN. Calcineurin B-like 3 and MTAN associate with each other in plant cells, they interact in vitro and in vivo, form a complex outside of the nucleus, colocalize mainly at the plasma membrane | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | localized at the plasma membrane, in the cytoplasm and in the nucleus of transgenic tobacco plants. Abundant in the membrane structure and weak in the cytoplasm of onion epidermal cells | Arabidopsis thaliana | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
- |
Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9T0I8 | - |
- |
Purification (Comment) | Organism |
---|---|
as a GST fusion form, by gel filtration | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cauline leaf | - |
Arabidopsis thaliana | - |
flower | highest level | Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
rosette leaf | - |
Arabidopsis thaliana | - |
stem | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-methylthioadenosine + phosphate | - |
Arabidopsis thaliana | adenine + 5-methylthio-D-ribose 1-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5'-methylthioadenosine nucleosidase | - |
Arabidopsis thaliana |
MTAN | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | none of MTAN deletion mutants interact with calcineurin B-like 3 | Arabidopsis thaliana |
physiological function | MTAN is involved in pollen development | Arabidopsis thaliana |