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Literature summary for 2.4.2.28 extracted from

  • Bacchi, C.J.; Goldberg, B.; Rattendi, D.; Gorrell, T.E.; Spiess, A.J.; Sufrin, J.R.
    Metabolic effects of a methylthioadenosine phosphorylase substrate analog on african trypanosomes (1999), Biochem. Pharmacol., 57, 89-96.
    View publication on PubMed
Search for more literature for 2.4.2.28

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5'-deoxy-5'-(hydroxyethylthio)adenosine + phosphate Trypanosoma brucei brucei trypanocidal substrate analogue adenine + 5-hydroxyethylthio-D-ribose 1-phosphate
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 ?
5'-deoxy-5'-(hydroxyethylthio)adenosine + phosphate Trypanosoma brucei brucei preincubation of cells with the substrate lead to 22-37% inhibition of spermidine synthesis from ornithine and 2-7fold increased cytosolic levels of S-adenosyl-L-methionine and S-adenosyl-L-homocysteine, and up to 8fold increased cytosolic level of 5'-methyladenosine adenine + 5-hydroxyethylthio-D-ribose 1-phosphate
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additional information Trypanosoma brucei brucei metabolic pathway, overview ?
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Organism

Organism UniProt Comment Textmining
Trypanosoma brucei brucei
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.048
-
 substrate 2-amino-5'-deoxy-5'-(hydroxyethylthio)adenosine Trypanosoma brucei brucei
0.17
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 substrate 5'-methyladenosine or 5'-deoxy-5'-(hydroxyethylthio)adenosine Trypanosoma brucei brucei
0.25
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 substrate 2-fluoro-5'-deoxy-5'-(hydroxyethylthio)adenosine Trypanosoma brucei brucei
0.365
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 substrate 5'-deoxy-5'-(hydroxyethylthio)adenosine, preincubation with substrate Trypanosoma brucei brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-5'-deoxy-5'-(hydroxyethylthio)adenosine + phosphate 28% activity compared to 5'-methyladenosine or 5'-deoxy-5'-(hydroxyethylthio)adenosine Trypanosoma brucei brucei 2-aminoadenosine + 5-hydroxyethylthio-D-ribose 1-phosphate
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 ?
2-fluoro-5'-deoxy-5'-(hydroxyethylthio)adenosine + phosphate 149% activity compared to 5'-methyladenosine or 5'-deoxy-5'-(hydroxyethylthio)adenosine Trypanosoma brucei brucei 2-fluoroadenosine + 5-hydroxyethylthio-D-ribose 1-phosphate
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 ?
5'-deoxy-5'-(hydroxyethylthio)adenosine + phosphate trypanocidal substrate analogue Trypanosoma brucei brucei adenine + 5-hydroxyethylthio-D-ribose 1-phosphate
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 ?
5'-deoxy-5'-(hydroxyethylthio)adenosine + phosphate preincubation of cells with the substrate lead to 22-37% inhibition of spermidine synthesis from ornithine and 2-7fold increased cytosolic levels of S-adenosyl-L-methionine and S-adenosyl-L-homocysteine, and up to 8fold increased cytosolic level of 5'-methyladenosine Trypanosoma brucei brucei adenine + 5-hydroxyethylthio-D-ribose 1-phosphate
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 ?
5'-methylthioadenosine + phosphate completely dependent on phosphate Trypanosoma brucei brucei adenine + 5-methylthio-D-ribose 1-phosphate
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 ?
additional information fluorine substitution at the C-2 position of the purine ring increases activity by 50%, whereas substitution with an amino group reduces activity to about one-third of the control Trypanosoma brucei brucei ?
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additional information metabolic pathway, overview Trypanosoma brucei brucei ?
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Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Trypanosoma brucei brucei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Trypanosoma brucei brucei