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Literature summary for 2.4.2.28 extracted from

  • Della Ragione, F.; Carteni-Farina, M.; Gragnaniello, V.; Schettino, M.I.; Zappia, V.
    Purification and characterization of 5'-deoxy-5'-methylthioadenosine phosphorylase from human placenta (1986), J. Biol. Chem., 261, 12324-12329.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol required Homo sapiens
SH-group reducing agents requirement Homo sapiens

General Stability

General Stability Organism
rapid inactivation in absence of reducing agents: 50% inactivation within 24 h at both 4°C and -20°C Homo sapiens
resistance to thermal inactivation is increased remarkably by addition of 5'-methylthioadenosine or phosphate Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
DTNB
-
Homo sapiens
iodoacetamide no inhibition Homo sapiens
iodoacetate no inhibition Homo sapiens
N-ethylmaleimide 5'-methylthioadenosine partly protects Homo sapiens
p-chloromercuribenzoic acid
-
Homo sapiens
SH-group blocking compounds
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
5'-methylthioadenosine
-
Homo sapiens
0.0055
-
5'-methylthioadenosine
-
Homo sapiens
0.008
-
5-methylthioribose 1-phosphate
-
Homo sapiens
0.023
-
adenine
-
Homo sapiens
0.32
-
phosphate
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32500
-
3 * 32500, SDS-PAGE with addition of 8 M urea and 1% 2-mercaptoethanol Homo sapiens
98000
-
gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.2
-
purified enzyme Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, 5 mM DTT, 50 mM potassium phosphate, pH 7.4, less than 10% loss of activity after 1 month Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-methylthioadenosine + phosphate
-
Homo sapiens adenine + 5-methylthio-D-ribose 1-phosphate
-
r

Subunits

Subunits Comment Organism
trimer 3 * 32500, SDS-PAGE with addition of 8 M urea and 1% 2-mercaptoethanol Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
resistance to thermal inactivation is increased remarkably by addition of 5'-methylthioadenosine or phosphate Homo sapiens
40 55 15 min, stable Homo sapiens
70
-
15 min, complete loss of activity Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pI: 5.5 Homo sapiens
7.1 7.6
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6.2 8.6 50% of activity maximum at pH 6.2 and pH 8.6 Homo sapiens