Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | required | Homo sapiens | |
SH-group reducing agents | requirement | Homo sapiens |
General Stability | Organism |
---|---|
rapid inactivation in absence of reducing agents: 50% inactivation within 24 h at both 4°C and -20°C | Homo sapiens |
resistance to thermal inactivation is increased remarkably by addition of 5'-methylthioadenosine or phosphate | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DTNB | - |
Homo sapiens | |
iodoacetamide | no inhibition | Homo sapiens | |
iodoacetate | no inhibition | Homo sapiens | |
N-ethylmaleimide | 5'-methylthioadenosine partly protects | Homo sapiens | |
p-chloromercuribenzoic acid | - |
Homo sapiens | |
SH-group blocking compounds | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
5'-methylthioadenosine | - |
Homo sapiens | |
0.0055 | - |
5'-methylthioadenosine | - |
Homo sapiens | |
0.008 | - |
5-methylthioribose 1-phosphate | - |
Homo sapiens | |
0.023 | - |
adenine | - |
Homo sapiens | |
0.32 | - |
phosphate | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32500 | - |
3 * 32500, SDS-PAGE with addition of 8 M urea and 1% 2-mercaptoethanol | Homo sapiens |
98000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.2 | - |
purified enzyme | Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, 5 mM DTT, 50 mM potassium phosphate, pH 7.4, less than 10% loss of activity after 1 month | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-methylthioadenosine + phosphate | - |
Homo sapiens | adenine + 5-methylthio-D-ribose 1-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 32500, SDS-PAGE with addition of 8 M urea and 1% 2-mercaptoethanol | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
resistance to thermal inactivation is increased remarkably by addition of 5'-methylthioadenosine or phosphate | Homo sapiens |
40 | 55 | 15 min, stable | Homo sapiens |
70 | - |
15 min, complete loss of activity | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 5.5 | Homo sapiens |
7.1 | 7.6 | - |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 8.6 | 50% of activity maximum at pH 6.2 and pH 8.6 | Homo sapiens |