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Literature summary for 2.4.2.26 extracted from
- Differences in gene expression of human xylosyltransferases and determination of acceptor specificities for various proteoglycans (2010), Biochem. Biophys. Res. Commun., 391, 685-691.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as glutathione-S-transferase fusion proteins containing putative or known GAG attachment sites of in vivo proteoglycans. Cloned into the XhoI and EcoRI digested pGEX-6P-1 vector and ligated by T4 DNA ligase. Expressed in Escherichia coli BL21 DE3 cells | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0023 | - |
serglycin | isoenzyme XT-II | Homo sapiens | |
| 0.0029 | - |
Thrombomodulin | isoenzyme XT-II | Homo sapiens | |
| 0.0032 | - |
neuroglycan C | isoenzyme XT-II | Homo sapiens | |
| 0.0034 | - |
collagen alpha2(IX) | isoenzyme XT-I | Homo sapiens | |
| 0.0035 | - |
syndecan-4 | isoenzyme XT-I | Homo sapiens | |
| 0.0064 | - |
bikunin | isoenzyme XT-II with mutant bikunin (sequence QEE-GSGRGQLV) | Homo sapiens | |
| 0.0074 | - |
syndecan-1 | isoenzyme XT-II | Homo sapiens | |
| 0.0077 | - |
bikunin | isoenzyme XT-II with mutant bikunin (sequence QEEEGSGHNMLV) | Homo sapiens | |
| 0.0082 | - |
testican-2 | isoenzyme XT-II | Homo sapiens | |
| 0.0085 | - |
bikunin | isoenzyme XT-II with mutant bikunin (sequence QEEESSGGGQLV) | Homo sapiens | |
| 0.0093 | - |
bFGF-peptide | isoenzyme XT-II | Homo sapiens | |
| 0.0093 | - |
bikunin | isoenzyme XT-II with mutant bikunin (sequence QEEEGSGSLALV) | Homo sapiens | |
| 0.0094 | - |
perlecan-3 | isoenzyme XT-II | Homo sapiens | |
| 0.0097 | - |
biglycan | isoenzyme XT-I | Homo sapiens | |
| 0.0099 | - |
bikunin | isoenzyme XT-I with mutant bikunin (sequence QEEERSGGGQLV) | Homo sapiens | |
| 0.0102 | - |
perlecan-2 | isoenzyme XT-II | Homo sapiens | |
| 0.0103 | - |
testican-2 | isoenzyme XT-I | Homo sapiens | |
| 0.0103 | - |
bikunin | isoenzyme XT-I with mutant bikunin (sequence QEEESSGGGQLV) | Homo sapiens | |
| 0.0104 | - |
L-APLP2 | isoenzyme XT-II | Homo sapiens |  |
| 0.0109 | - |
glypican-1 | isoenzyme XT-II | Homo sapiens | |
| 0.0111 | - |
serglycin | isoenzyme XT-I | Homo sapiens | |
| 0.0122 | - |
syndecan | isoenzyme XT-II | Homo sapiens | |
| 0.0123 | - |
bikunin | isoenzyme XT-I with mutant bikunin (sequence QEE-GSGGGQLV) | Homo sapiens | |
| 0.0125 | - |
bikunin | isoenzyme XT-I with mutant bikunin (sequence QEEEGSGHNMLV) | Homo sapiens | |
| 0.0127 | - |
versican-beta | isoenzyme XT-II | Homo sapiens | |
| 0.0131 | - |
L-APP | XT-I | Homo sapiens | |
| 0.0133 | - |
perlecan-1 | isoenzyme XT-I | Homo sapiens | |
| 0.0133 | - |
bikunin | isoenzyme XT-I with mutant bikunin (sequence QEEEGSSCGQLV) | Homo sapiens | |
| 0.0134 | - |
L-APLP2 | isoenzyme XT-I | Homo sapiens | |
| 0.0137 | - |
bFGF-peptide | isoenzyme XT-I | Homo sapiens | |
| 0.0137 | - |
bikunin | isoenzyme XT-II with mutant bikunin (sequence QEEEGSGRRALV) | Homo sapiens | |
| 0.0141 | - |
syndecan-4 | isoenzyme XT-II | Homo sapiens | |
| 0.0145 | - |
perlecan-2 | isoenzyme XT-I | Homo sapiens | |
| 0.0146 | - |
CD44 | isoenzyme XT-I | Homo sapiens | |
| 0.0146 | - |
versican-beta | isoenzyme XT-I | Homo sapiens | |
| 0.015 | - |
bikunin | isoenzyme XT-II with mutant bikunin (sequence QEEEGSGGRSVLV) | Homo sapiens | |
| 0.0151 | - |
bikunin | isoenzyme XT-I with mutant bikunin (sequence QEEEGSGCGQLV) | Homo sapiens | |
| 0.0153 | - |
bikunin | isoenzyme XT-I with mutant bikunin (sequence QEEEGSSGGQLV) | Homo sapiens | |
| 0.0166 | - |
phosphacan | isoenzyme XT-I | Homo sapiens | |
| 0.0171 | - |
glypican-1 | isoenzyme XT-I | Homo sapiens | |
| 0.0178 | - |
syndecan | isoenzyme XT-I | Homo sapiens | |
| 0.0179 | - |
bamcan | isoenzyme XT-II | Homo sapiens | |
| 0.0182 | - |
Thrombomodulin | isoenzyme XT-I | Homo sapiens | |
| 0.0187 | - |
perlecan-3 | isoenzyme XT-I | Homo sapiens | |
| 0.0188 | - |
Aggrecan | isoenzyme XT-I | Homo sapiens | |
| 0.0198 | - |
syndecan-1 | isoenzyme XT-I | Homo sapiens | |
| 0.0199 | - |
bikunin | isoenzyme XT-I with mutant bikunin (sequence QEEEGSGSLALV) | Homo sapiens | |
| 0.02 | - |
bikunin | isoenzyme XT-I with mutant bikunin (sequence QKEEGSGGGQLV) | Homo sapiens | |
| 0.0205 | - |
bikunin | isoenzyme XT-I with mutant bikunin (sequence QEE-GSGRGQLV) | Homo sapiens | |
| 0.0208 | - |
betaglycan-1 | isoenzyme XT-I | Homo sapiens | |
| 0.0215 | - |
bikunin | isoenzyme XT-II with mutant bikunin (sequence QEEEGSGCYKLV) | Homo sapiens | |
| 0.0222 | - |
bamcan | isoenzyme XT-I | Homo sapiens | |
| 0.0227 | - |
bikunin | isoenzyme XT-II with mutant bikunin (sequence QEEEGSGCGQLV) | Homo sapiens | |
| 0.0236 | - |
neuroglycan C | isoenzyme XT-I | Homo sapiens | |
| 0.0242 | - |
bikunin | isoenzyme XT-II with mutant bikunin (sequence QEEEGSGRHLLV) | Homo sapiens | |
| 0.0243 | - |
bikunin | isoenzyme XT-I with mutant bikunin (sequence QEEESSGRGQLV) | Homo sapiens | |
| 0.0251 | - |
bikunin | isoenzyme XT-I with mutant bikunin (sequence QEEEGSGGRSVLV) | Homo sapiens | |
| 0.0274 | - |
bikunin | isoenzyme XT-I with wild-type bikunin (sequence QEEEGSGGGQLV) | Homo sapiens | |
| 0.0288 | - |
bikunin | isoenzyme XT-I with mutant bikunin (sequence QEEEGSTSLV) | Homo sapiens | |
| 0.0301 | - |
bikunin | isoenzyme XT-I with mutant bikunin (sequence QEEEGSGCYKLV) | Homo sapiens | |
| 0.0318 | - |
bikunin | isoenzyme XT-I with mutant bikunin (sequence QLTTGSGGGQLV) | Homo sapiens | |
| 0.0356 | - |
bikunin | isoenzyme XT-II with mutant bikunin (sequence QKEEGSGGGQLV) | Homo sapiens | |
| 0.0462 | - |
bikunin | isoenzyme XT-II with wild-type bikunin (sequence QEEEGSGGGQLV) | Homo sapiens | |
| 0.0463 | - |
bikunin | isoenzyme XT-II | Homo sapiens | |
| 0.0508 | - |
bikunin | isoenzyme XT-II with mutant bikunin (sequence QEEEGSGCRVLV) | Homo sapiens | |
| 0.0543 | - |
bikunin | isoenzyme XT-I with mutant bikunin (sequence QEEEGSGCRVLV) | Homo sapiens | |
| 0.0572 | - |
bikunin | isoenzyme XT-I with mutant bikunin (sequence QEEEGSGRHLLV) | Homo sapiens | |
| 0.0586 | - |
bikunin | isoenzyme XT-I with mutant bikunin (sequence QEEEGSGRRALV) | Homo sapiens | |
| 0.0656 | - |
bikunin | isoenzyme XT-I with mutant bikunin (sequence QPQIGSGGGQLV) | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by affinity chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| A-431 cell | XT-I mRNA is not expressed but XT-II mRNA | Homo sapiens | - |
| A-549 cell | XT-II mRNA expression is higher than for XT-I. Very low XT-I mRNA expression | Homo sapiens | - |
| BM-1604 cell | XT-I mRNA is not expressed but XT-II mRNA | Homo sapiens | - |
| BT-474 cell | highest XT-II expression levels, low XT-I mRNA expression | Homo sapiens | - |
| CAPAN-1 cell | XT-II mRNA expression is higher than for XT-I | Homo sapiens | - |
| CAPAN-2 cell | XT-I mRNA is not expressed but XT-II mRNA | Homo sapiens | - |
| Chang cell | XT-II mRNA expression is higher than for XT-I. Very low XT-I mRNA expression | Homo sapiens | - |
| EAhy 926 cell | XT-II mRNA expression is higher than for XT-I. Very low XT-I mRNA expression | Homo sapiens | - |
| ECV-304 cell | XT-II mRNA expression is higher than for XT-I. Very low XT-I mRNA expression | Homo sapiens | - |
| EFO-27 cell | XT-II mRNA expression is higher than for XT-I. Low XT-I mRNA expression | Homo sapiens | - |
| HEK-293 cell | XT-II mRNA expression is higher than for XT-I. Very low XT-I mRNA expression | Homo sapiens | - |
| HeLa cell | XT-I mRNA is not expressed but XT-II mRNA | Homo sapiens | - |
| Hep-G2 cell | XT-II mRNA expression is higher than for XT-I. Very low XT-I mRNA expression | Homo sapiens | - |
| HT-1080 cell | XT-I mRNA is not expressed but XT-II mRNA | Homo sapiens | - |
| JAR cell | XT-I mRNA expression is higher than for XT-II | Homo sapiens | - |
| K-562 cell | XT-I mRNA is not expressed but XT-II mRNA | Homo sapiens | - |
| LNCaP cell | XT-I mRNA is not expressed but XT-II mRNA | Homo sapiens | - |
| MCF-7 cell | XT-II mRNA expression is higher than for XT-I | Homo sapiens | - |
| additional information | equal expression levels of XT-I and XT-II in 1301 cells, WERI-RB-1 cells and HS-27 cells. In 3051/80 cells, SW-982 cells, MHH-ES-1 cells, hTERT-BJ1 cells and EFO-21 cells, XT-II mRNA expression is higher than for XT-I. In 23132/87 cells, XT-I mRNA expression is higher than for XT-II. In NCI-H510A cells, highest XT-I expression levels, XT-I mRNA expression is higher than for XT-II | Homo sapiens | - |
| RPMI-2650 cell | XT-II mRNA expression is higher than for XT-I. Very low XT-I mRNA expression | Homo sapiens | - |
| SAOS-2 cell | equal expression levels of XT-I and XT-II | Homo sapiens | - |
| SW-1353 cell | equal expression levels of XT-I and XT-II | Homo sapiens | - |
| THP-1 cell | XT-I mRNA expression is higher than for XT-II | Homo sapiens | - |
| Y-79 cell | equal expression levels of XT-I and XT-II | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-D-xylose + aggrecan | - |
Homo sapiens | UDP + ? | - |
? | |
| UDP-D-xylose + bamcan | - |
Homo sapiens | UDP + ? | - |
? | |
| UDP-D-xylose + betaglycan-1 | - |
Homo sapiens | UDP + ? | - |
? | |
| UDP-D-xylose + bFGF-peptide | - |
Homo sapiens | UDP + ? | - |
? | |
| UDP-D-xylose + biglycan | - |
Homo sapiens | UDP + D-xylosyl-biglycan | - |
? | |
| UDP-D-xylose + bikunin | - |
Homo sapiens | UDP + ? | - |
? | |
| UDP-D-xylose + CD44 | - |
Homo sapiens | UDP + ? | - |
? | |
| UDP-D-xylose + collagen alpha2(IX) | - |
Homo sapiens | UDP + ? | - |
? | |
| UDP-D-xylose + glypican-1 | - |
Homo sapiens | UDP + ? | - |
? | |
| UDP-D-xylose + L-APLP2 | - |
Homo sapiens | UDP + ? | - |
? | |
| UDP-D-xylose + L-APP | - |
Homo sapiens | UDP + ? | - |
? | |
| UDP-D-xylose + neuroglycan C | - |
Homo sapiens | UDP + ? | - |
? | |
| UDP-D-xylose + perlecan-1 | - |
Homo sapiens | UDP + ? | - |
? | |
| UDP-D-xylose + perlecan-2 | - |
Homo sapiens | UDP + ? | - |
? | |
| UDP-D-xylose + perlecan-3 | - |
Homo sapiens | UDP + ? | - |
? | |
| UDP-D-xylose + phosphacan | - |
Homo sapiens | UDP + ? | - |
? | |
| UDP-D-xylose + serglycin | - |
Homo sapiens | UDP + ? | - |
? | |
| UDP-D-xylose + syndecan | - |
Homo sapiens | UDP + ? | - |
? | |
| UDP-D-xylose + syndecan-1 | - |
Homo sapiens | UDP + ? | - |
? | |
| UDP-D-xylose + syndecan-4 | - |
Homo sapiens | UDP + ? | - |
? | |
| UDP-D-xylose + testican-2 | - |
Homo sapiens | UDP + ? | - |
? | |
| UDP-D-xylose + thrombomodulin | - |
Homo sapiens | UDP + ? | - |
? | |
| UDP-D-xylose + versican-beta | - |
Homo sapiens | UDP + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| XT-I | - |
Homo sapiens |
| XT-II | - |
Homo sapiens |
| xylosyltransferase I | - |
Homo sapiens |
| xylosyltransferase II | - |
Homo sapiens |
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