Cloned (Comment) | Organism |
---|---|
expressed in xylosyltransferase-deficient pgsA-745 CHO cells | Homo sapiens |
expression in Pichia pastoris. Transfection of the xylosyltransferase-deficient Chinese hamster ovary mutant pgsA-745 with XT-II coding cDNA completely restores glycosaminoglycan biosynthesis | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0052 | - |
biotin-NH-QEEEGSGGGQKK(5-fluorescein)-CONH2 | - |
Homo sapiens | |
0.0052 | - |
biotin-NHQEEEGSGGGQKK(5-fluorescein)-CONH2 | recombinant enzyme | Homo sapiens | |
0.022 | - |
bikunin | - |
Homo sapiens | |
0.022 | - |
bikunin | recombinant enzyme | Homo sapiens | |
0.062 | - |
fibroblast growth factor 2 | recombinant enzyme | Homo sapiens | |
0.677 | - |
silk fibroin | - |
Homo sapiens | |
0.677 | - |
silk fibroin | recombinant enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secreted into the culture supernatant | Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | xylosyltransferase II is involved in the biosynthesis of the uniform tetrasaccharide linkage region in chondroitin sulfate and heparan sulfate proteoglycans | ? | - |
? | |
additional information | Homo sapiens | XT-II initiates the biosynthesis of both heparan sulfate and chondroitin sulfate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | Q9H1B5 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture supernatant | - |
Homo sapiens | - |
kidney | XT-I and XT-II are highly expressed in kidney | Homo sapiens | - |
liver | XT-II is highly expressed in liver, no XT-I mRNA is detectable in liver | Homo sapiens | - |
lung | XT-II is highly expressed in lung | Homo sapiens | - |
testis | XT-I is highly expressed in testes | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | xylosyltransferase II is involved in the biosynthesis of the uniform tetrasaccharide linkage region in chondroitin sulfate and heparan sulfate proteoglycans | Homo sapiens | ? | - |
? | |
additional information | XT-II initiates the biosynthesis of both heparan sulfate and chondroitin sulfate | Homo sapiens | ? | - |
? | |
UDP-D-xylose + bikunin | - |
Homo sapiens | UDP + D-xylosyl-bikunin | - |
? | |
UDP-D-xylose + bikunin | - |
Homo sapiens | ? | - |
? | |
UDP-D-xylose + biotin-NH-QEEEGSGGGQKK(5-fluorescein)-CONH2 | best substrate known so far for XT-IImediated xylosylation | Homo sapiens | UDP + biotin-NH-QEEEGS(D-xylosyl)GGGQKK(5-fluorescein)-CONH2 | - |
? | |
UDP-D-xylose + biotin-NHQEEEGSGGGQKK(5-fluorescein)-CONH2 | biotin-NHQEEEGSGGGQKK(5-fluorescein)-CONH2is the best acceptor for isozyme XT-II | Homo sapiens | UDP + biotin-NHQEEEG-(D-xylosyl)SGGGQKK(5-fluorescein)-CONH2 | - |
? | |
UDP-D-xylose + fibroblast growth factor 2 | - |
Homo sapiens | ? | - |
? | |
UDP-D-xylose + silk fibroin | silk fibroin is a low affinity acceptor for XT-II | Homo sapiens | UDP + silk fibroin with xylosylserine | - |
? | |
UDP-D-xylose + silk fibroin | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UDP-D-xylose:proteoglycan core protein beta-D-xylosyltransferase | - |
Homo sapiens |
XT-II | - |
Homo sapiens |
xylosyltransferase II | - |
Homo sapiens |