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Literature summary for 2.4.2.26 extracted from

  • Mueller, S.; Disse, J.; Schoettler, M.; Schoen, S.; Prante, C.; Brinkmann, T.; Kuhn, J.; Kleesiek, K.; Goetting, C.
    Human xylosyltransferase I and N-terminal truncated forms: functional characterization of the core enzyme (2006), Biochem. J., 394, 163-171.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.2.26

Application

Application Comment Organism
diagnostics the enzyme is a diagnostic marker of an enhanced proteoglycan biosynthesis Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
soluble mutant enzymes of human XT-I with deletions at the N-terminal domain are expressed in insect cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
C276A mutation results in a nearly inactive enzyme Homo sapiens
DELTA1-184 KM-value for bikunin is identical to wild-type value, Vmax is 1.2fold higher than wild-type value Homo sapiens
DELTA1-213 KM-value for bikunin is 1.6fold higher than wild-type value, Vmax is 1.1fold higher than wild-type value Homo sapiens
DELTA1-260 KM-value for bikunin is 1.6fold higher than wild-type value, Vmax is identical to wild-type value Homo sapiens
DELTA1-266 KM-value for bikunin is 5.6fold higher than wild-type value, Vmax is 1.6fold lower than wild-type value Homo sapiens
DELTA1-272 KM-value for bikunin is 6fold higher than wild-type value, Vmax is 1.3fold lower than wild-type value Homo sapiens
DELTA1-273 more than 98% of activity Homo sapiens
DELTA261-272 inactive mutant enzyme Homo sapiens
DELTA721-726 inactive mutant enzyme Homo sapiens
E263A KM-value for bikunin is 1.4fold higher than wild-type value, Vmax is 2fold higher than wild-type value Homo sapiens
K262A KM-value for bikunin is 1.2fold higher than wild-type value, Vmax is 1.7fold higher than wild-type value Homo sapiens
K272A KM-value for bikunin is 1.4fold higher than wild-type value, Vmax is 1.7fold higher than wild-type value Homo sapiens
additional information truncation of 266, 272 and 273 amino acids in the N-terminal region results in a 70, 90 and above 98% loss in catalytic activity. Deletion of the single 12 amino acid motif G261KEAISALSRAK272 leads to a loss-of-function xylosyltransferase I mutant. Heparin binding is slightly altered in mutants lacking 289 or 568 amino acids, but deletion of the potential heparin-binding motif P721KKVFKI727 does not lead to a loss of heparin binding capacity Homo sapiens
R270A KM-value for bikunin is 1.2fold higher than wild-type value, Vmax is 1.4fold higher than wild-type value Homo sapiens
S266A KM-value for bikunin is 1.4fold higher than wild-type value, Vmax is 1.3fold higher than wild-type value Homo sapiens
S269A KM-value for bikunin is 1.3fold higher than wild-type value, Vmax is 1.1fold higher than wild-type value Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
 bikunin 37°C, pH 6.5 Homo sapiens
0.0009
-
 bikunin 37°C, pH 6.5, mutant enzyme DELTA1-184 Homo sapiens
0.0011
-
 bikunin 37°C, pH 6.5, mutant enzyme K262A Homo sapiens
0.0011
-
 bikunin 37°C, pH 6.5, mutant enzyme R270A Homo sapiens
0.0012
-
 bikunin 37°C, pH 6.5, mutant enzyme S269A Homo sapiens
0.0013
-
 bikunin 37°C, pH 6.5, mutant enzyme E263A Homo sapiens
0.0013
-
 bikunin 37°C, pH 6.5, mutant enzyme K272A Homo sapiens
0.0013
-
 bikunin 37°C, pH 6.5, mutant enzyme S266A Homo sapiens
0.0014
-
 bikunin 37°C, pH 6.5, mutant enzyme DELTA1-213 Homo sapiens
0.0014
-
 bikunin 37°C, pH 6.5, mutant enzyme DELTA1-260 Homo sapiens
0.005
-
 bikunin 37°C, pH 6.5, mutant enzyme DELTA1-266 Homo sapiens
0.0054
-
 bikunin 37°C, pH 6.5, mutant enzyme DELTA1-272 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-xylose + [protein]-L-serine Homo sapiens initiates the biosynthesis of the glycosaminoglycan linkage region UDP + [protein]-3-O-(beta-D-xylosyl)-L-serine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-xylose + [protein]-L-serine initiates the biosynthesis of the glycosaminoglycan linkage region Homo sapiens UDP + [protein]-3-O-(beta-D-xylosyl)-L-serine
-
 ?
UDP-D-xylose + bikunin
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
xylosyltransferase I
-
 Homo sapiens