Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithioerythritol | stimulates enzyme activity up to 35%, optimum concentration: 6 mM | Petroselinum crispum |
General Stability | Organism |
---|---|
albumin, 0.1 mg, significant stabilization | Petroselinum crispum |
solution of enzyme after hydroxylapatite chromatography in sodium phosphate buffer, pH 7.0, rapidly loses activity upon freezing | Petroselinum crispum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
apigenin-7-glucoside | - |
Petroselinum crispum | |
apiin | - |
Petroselinum crispum | |
Ca2+ | - |
Petroselinum crispum | |
Chrysoeriol-7-glucoside | - |
Petroselinum crispum | |
Co2+ | - |
Petroselinum crispum | |
iodoacetamide | at 1 mM causes an inhibition of 55%, reversed by cysteine | Petroselinum crispum | |
Mg2+ | - |
Petroselinum crispum | |
Mn2+ | - |
Petroselinum crispum | |
additional information | no effect: EDTA, NH4+, K+ | Petroselinum crispum | |
p-chloromercuribenzoate | at 1 mM inhibits the reaction completely | Petroselinum crispum | |
UDP | - |
Petroselinum crispum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.066 | - |
apigenin-7-glucoside | - |
Petroselinum crispum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Petroselinum crispum | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
gel filtration | Petroselinum crispum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDPapiose + 7-O-(beta-D-glucosyl)-apigenin | Petroselinum crispum | biosynthesis of apiin | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Petroselinum crispum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purified 123fold by protamine sulfate and ammonium sulfate precipitation and chromatography on DEAE-cellulose, SephadexG-100 and hydroxylapatite | Petroselinum crispum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Petroselinum crispum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Petroselinum crispum |
Storage Stability | Organism |
---|---|
-20°C, Tris-HCl, dithiothreitol, protein concentration 0.5-1 mg/ml | Petroselinum crispum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | overview: 7-O-beta-glucosides of a number of flavonoids and of 4-substituted phenols can act as acceptors, not: flavonol-3-glucoside, flavonol-7-glucoside, apigenin-8-C-glucoside, aglycones of flavonoids, glucose | Petroselinum crispum | ? | - |
? | |
UDPapiose + 7-O-(beta-D-glucosyl)-apigenin | specific for UDPapiose as glycosyl donor | Petroselinum crispum | UDP + 7-O-beta-D-apiofuranosyl-1,2-beta-D-glucosyl-apigenin | i.e. apiin | ? | |
UDPapiose + 7-O-(beta-D-glucosyl)-apigenin | biosynthesis of apiin | Petroselinum crispum | ? | - |
? | |
UDPapiose + biochanin A | i.e. 5,7-dihydroxy-4'-methoxyisoflavone-7-glucoside | Petroselinum crispum | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Petroselinum crispum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
higher apiin yield in Tris-HCl than in phosphate buffer | Petroselinum crispum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | highest stability | Petroselinum crispum |