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Literature summary for 2.4.2.25 extracted from

  • Ortmann, R.; Sutter, A.; Grisebach, H.
    Purification and properties of udpapiose: 7-O-(beta-D-glucosyl)-flavone apiosyltransferase from cell suspension cultures of parsley (1972), Biochim. Biophys. Acta, 289, 293-302.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithioerythritol stimulates enzyme activity up to 35%, optimum concentration: 6 mM Petroselinum crispum

General Stability

General Stability Organism
albumin, 0.1 mg, significant stabilization Petroselinum crispum
solution of enzyme after hydroxylapatite chromatography in sodium phosphate buffer, pH 7.0, rapidly loses activity upon freezing Petroselinum crispum

Inhibitors

Inhibitors Comment Organism Structure
apigenin-7-glucoside
-
Petroselinum crispum
apiin
-
Petroselinum crispum
Ca2+
-
Petroselinum crispum
Chrysoeriol-7-glucoside
-
Petroselinum crispum
Co2+
-
Petroselinum crispum
iodoacetamide at 1 mM causes an inhibition of 55%, reversed by cysteine Petroselinum crispum
Mg2+
-
Petroselinum crispum
Mn2+
-
Petroselinum crispum
additional information no effect: EDTA, NH4+, K+ Petroselinum crispum
p-chloromercuribenzoate at 1 mM inhibits the reaction completely Petroselinum crispum
UDP
-
Petroselinum crispum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.066
-
apigenin-7-glucoside
-
Petroselinum crispum

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Petroselinum crispum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
gel filtration Petroselinum crispum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDPapiose + 7-O-(beta-D-glucosyl)-apigenin Petroselinum crispum biosynthesis of apiin ?
-
?

Organism

Organism UniProt Comment Textmining
Petroselinum crispum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified 123fold by protamine sulfate and ammonium sulfate precipitation and chromatography on DEAE-cellulose, SephadexG-100 and hydroxylapatite Petroselinum crispum

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Petroselinum crispum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Petroselinum crispum

Storage Stability

Storage Stability Organism
-20°C, Tris-HCl, dithiothreitol, protein concentration 0.5-1 mg/ml Petroselinum crispum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information overview: 7-O-beta-glucosides of a number of flavonoids and of 4-substituted phenols can act as acceptors, not: flavonol-3-glucoside, flavonol-7-glucoside, apigenin-8-C-glucoside, aglycones of flavonoids, glucose Petroselinum crispum ?
-
?
UDPapiose + 7-O-(beta-D-glucosyl)-apigenin specific for UDPapiose as glycosyl donor Petroselinum crispum UDP + 7-O-beta-D-apiofuranosyl-1,2-beta-D-glucosyl-apigenin i.e. apiin ?
UDPapiose + 7-O-(beta-D-glucosyl)-apigenin biosynthesis of apiin Petroselinum crispum ?
-
?
UDPapiose + biochanin A i.e. 5,7-dihydroxy-4'-methoxyisoflavone-7-glucoside Petroselinum crispum ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Petroselinum crispum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
higher apiin yield in Tris-HCl than in phosphate buffer Petroselinum crispum

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 highest stability Petroselinum crispum