Cloned (Comment) | Organism |
---|---|
expressed in tobacco BY2 cells | Populus trichocarpa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.27 | - |
xylotetraose | co-substrate: UDP-D-xylose, Km determined in microsomes co-expressing PtrGT43B and PtrGT43C. pH and temperature not specified in the publication | Populus trichocarpa | |
5.27 | - |
UDP-D-xylose | co-substrate: xylotetraose, Km determined in microsomes co-expressing PtrGT43B and PtrGT43C. pH and temperature not specified in the publication | Populus trichocarpa | |
5.9 | - |
xylotetraose | co-substrate: UDP-D-xylose, Km determined in microsomes from poplar stems. pH and temperature not specified in the publication | Populus trichocarpa | |
5.9 | - |
UDP-D-xylose | co-substrate: xylotetraose, Km determined in microsomes from poplar stems. pH and temperature not specified in the publication | Populus trichocarpa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Populus trichocarpa | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus trichocarpa | B9HH87 | - |
- |
Populus trichocarpa | B9IJ10 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-xylose + xylotetraose | microsomes isolated from transgenic BY2 cells are tested for XylT activity using xylotetraose (Xyl4) as an acceptor and UDP-xylose as a donor. XylT activity can not use the xylose monomer as an acceptor. Xylosyl residues are readily transferred onto xylooligomers ranging from 2 to 6 residues, resulting in the production of xylooligosaccharides with up to 11 xylosyl residues | Populus trichocarpa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PtrGT43B | - |
Populus trichocarpa |
PtrGT43C | - |
Populus trichocarpa |
General Information | Comment | Organism |
---|---|---|
malfunction | microsomes from transgenic tobacco BY2 cells coexpressing PtrGT43B and PtrGT43C exhibit a high XylT activity capable of generating beta-(1,4)-linked xylooligosaccharides, whereas little XylT activity is detected in microsomes with expression of PtrGT43B or PtrGT43C alone | Populus trichocarpa |
physiological function | PtrGT43B and PtrGT43C, are xylan XylTs involved in wood formation | Populus trichocarpa |