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Literature summary for 2.4.2.24 extracted from

  • Lee, C.; Zhong, R.; Ye, Z.
    Biochemical characterization of xylan xylosyltransferases involved in wood formation in poplar (2012), Plant Signal. Behav., 7, 332-337.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in tobacco BY2 cells Populus trichocarpa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.27
-
xylotetraose co-substrate: UDP-D-xylose, Km determined in microsomes co-expressing PtrGT43B and PtrGT43C. pH and temperature not specified in the publication Populus trichocarpa
5.27
-
UDP-D-xylose co-substrate: xylotetraose, Km determined in microsomes co-expressing PtrGT43B and PtrGT43C. pH and temperature not specified in the publication Populus trichocarpa
5.9
-
xylotetraose co-substrate: UDP-D-xylose, Km determined in microsomes from poplar stems. pH and temperature not specified in the publication Populus trichocarpa
5.9
-
UDP-D-xylose co-substrate: xylotetraose, Km determined in microsomes from poplar stems. pH and temperature not specified in the publication Populus trichocarpa

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Populus trichocarpa
-
-

Organism

Organism UniProt Comment Textmining
Populus trichocarpa B9HH87
-
-
Populus trichocarpa B9IJ10
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-D-xylose + xylotetraose microsomes isolated from transgenic BY2 cells are tested for XylT activity using xylotetraose (Xyl4) as an acceptor and UDP-xylose as a donor. XylT activity can not use the xylose monomer as an acceptor. Xylosyl residues are readily transferred onto xylooligomers ranging from 2 to 6 residues, resulting in the production of xylooligosaccharides with up to 11 xylosyl residues Populus trichocarpa ?
-
?

Synonyms

Synonyms Comment Organism
PtrGT43B
-
Populus trichocarpa
PtrGT43C
-
Populus trichocarpa

General Information

General Information Comment Organism
malfunction microsomes from transgenic tobacco BY2 cells coexpressing PtrGT43B and PtrGT43C exhibit a high XylT activity capable of generating beta-(1,4)-linked xylooligosaccharides, whereas little XylT activity is detected in microsomes with expression of PtrGT43B or PtrGT43C alone Populus trichocarpa
physiological function PtrGT43B and PtrGT43C, are xylan XylTs involved in wood formation Populus trichocarpa