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Literature summary for 2.4.2.24 extracted from

  • Lee, C.; ONeill, M.A.; Tsumuraya, Y.; Darvill, A.G.; Ye, Z.
    The irregular xylem9 mutant is deficient in xylan xylosyltransferase activity (2007), Plant Cell Physiol., 48, 1624-1634.
    View publication on PubMed
Search for more literature for 2.4.2.24

Activating Compound

Activating Compound Comment Organism Structure
UDP-D-glucuronic acid 30% increased XylT activity in microsomal fractions of stems from wild-type plants possibly due to increased solubility of xylooligomers Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10.6
-
 (1,4-beta-D-xylopyranose)6 microsomal fractions of stems from wild-type plants Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome activity in microsomal fractions enriched with Golgi marker enzyme inosin diphosphatase Arabidopsis thaliana
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arabidopsis thaliana xylosyltransferase (XylT) activity, biosynthesis of xylan, a major component of dicot wood ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
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 ecotype Columbia: wild-type plant, mutant plant irx9 (T-DNA insertion (SALK_058238) in the irx9 mutant disrupts an IRX9 exon that encodes part of the putative catalytic domain), or mutant plant irx9 complemented with IRX9 wild-type protein
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Purification (Commentary)

Purification (Comment) Organism
activity in microsomal fractions from stems, roots, leaves and seedlings, homogenized with mortar and pestle in 50 mM HEPES pH7.3, 0.4 M sucrose followed by differential centrifugation, storage at -80°C Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
stem XylT activity in microsomal fraction only in presence of exogenous Xyl(6), wild-type plant Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 substantial decrease in XylT activity and deficiency in elongation of xylooligosaccharides in microsomal fractions of stems from irx9 mutant plants Arabidopsis thaliana
0.0000218
-
 with respect to (1,4-beta-D-xylopyranose)6, microsomal fractions of stems from wild-type plants Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information xylosyltransferase (XylT) activity, biosynthesis of xylan, a major component of dicot wood Arabidopsis thaliana ?
-
 ?
additional information synthesis of xylooligosaccharides from Xyl(6)-anthranilic acid by XylT activity in microsomal fractions of stems from irx9 mutant plants: after up to 5 h mixture of Xyl(7) to Xyl(9) only, Xyl(7) predominates Arabidopsis thaliana ?
-
 ?
additional information synthesis of xylooligosaccharides from Xyl(6)-anthranilic acid by XylT activity in microsomal fractions of stems from wild-type plants: after 2 h Xyl(7) to Xyl(9) predominate, after 5 h increased proportion of Xyl(10) to Xyl(12) Arabidopsis thaliana ?
-
 ?
additional information Xyl(1)-anthranilic acid is not acceptor for XylT activity in microsomal fractions of stems from wild-type plants Arabidopsis thaliana ?
-
 ?
additional information Xyl(2)-anthranilic acid is a weak acceptor for XylT activity in microsomal fractions of stems from wild-type plants Arabidopsis thaliana ?
-
 ?
additional information Xyl(3)-anthranilic acid to Xyl(6)-anthranilic acid are readily accepted by XylT activity in microsomal fractions of stems from wild-type plants Arabidopsis thaliana ?
-
 ?
additional information Xyl(4)-anthranilic acid is acceptor for synthesis of xylooligosaccharides of sizes from Xyl(5) to Xyl(11) by XylT activity in microsomal fractions of stems from wild-type plants within 2 h, prolonged incubation time (5-8 h) leads to increased proportion of Xyl(9) to Xyl(11) but not to synthesis of longer xylooligosaccharides Arabidopsis thaliana ?
-
 ?
UDP-D-xylose + ((1,4-beta-D-xylooligosaccharide)n)-anthranilic acid 0.1 mM UDP-xylose + 0.5 mM anthranilic acid (AA) labelled xylooligosaccharides (Xyl(2) to Xyl(6)) as acceptor, 200 microgram microsomes, 21°C, pH 6.8, in presence of 5 mM MnCl2, 1 mM dithiothreitol, 0.5% Triton X-100 Arabidopsis thaliana UDP + ((1,4-beta-D-xylooligosaccharide)n+1)-anthranilic acid xylooligosaccharides (up to Xyl(12)) purified by HPLC and analysed by MALDI-TOF mass spectrometry ?
UDP-D-xylose + (1,4-beta-D-xylooligosaccharide)n 0.1 mM radiolabelled UDP-D-xylose + 0.2 microgram/microlitre (1,4-beta-D-xylopyranose)6, 100 microgram microsomes, 21°C, pH 6.8, in presence of 5 mM MnCl2, 1 mM dithiothreitol, 0.5% Triton X-100, reaction dependent on time and protein concentration Arabidopsis thaliana UDP + (1,4-beta-D-xylooligosaccharide)n+1 reduction in XylT activity in microsomal fraction of stems from irx9 mutant plants compared to wild-type plants in presence of exogenous substrates can be abolished by complementation with wild-type IRX9 gene, product quantification by scintillation counting ?
UDP-D-xylose + (1,4-beta-D-xylopyranose)6
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 Arabidopsis thaliana UDP + (1,4-beta-D-xylopyranose)7
-
 ?

Synonyms

Synonyms Comment Organism
irregular xylem9
-
 Arabidopsis thaliana
IRX9
-
 Arabidopsis thaliana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 20 microsomal fractions of stems from wild-type plants Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 microsomal fractions of stems from wild-type plants Arabidopsis thaliana