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Literature summary for 2.4.2.24 extracted from

  • Urahara, T.; Tsuchiya, K.; Kotake, T.; Tohno-oka, T.; Komae, K.; Kawada, N.; Tsumuraya, Y.
    A beta-(1->4)-xylosyltransferase involved in the synthesis of arabinoxylans in developing barley endosperms (2004), Physiol. Plant., 122, 169-180.
No PubMed abstract available
Search for more literature for 2.4.2.24

Activating Compound

Activating Compound Comment Organism Structure
3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate i.e. CHAPS, slight activation Hordeum vulgare
Brij-35 slight activation Hordeum vulgare
additional information enzyme requires detergents for activity Hordeum vulgare
Triton X-100 high activation at 0.5% Hordeum vulgare
Triton X-102
-
 Hordeum vulgare
Tween 20
-
 Hordeum vulgare
Zwittergent 3-16
-
 Hordeum vulgare

Inhibitors

Inhibitors Comment Organism Structure
Al3+
-
 Hordeum vulgare
Ca2+
-
 Hordeum vulgare
Cu2+
-
 Hordeum vulgare
Fe3+
-
 Hordeum vulgare
Hg2+
-
 Hordeum vulgare
n-octyl-beta-D-glucoside 50% inhibition Hordeum vulgare
sodium cholate complete inhibition Hordeum vulgare
sodium deoxycholate complete inhibition Hordeum vulgare
Zn2+
-
 Hordeum vulgare

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1
-
 pyridylaminated beta-(1,4)-xylotriose pH 6.8, 20°C Hordeum vulgare

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Hordeum vulgare 16020
-
microsome
-
 Hordeum vulgare
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ enzyme requires Mg2+ or Mn2+ for activity, activates Hordeum vulgare
Mn2+ enzyme requires Mg2+ or Mn2+ for activity, high activation at 5 mM MnCl2 Hordeum vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Hordeum vulgare metabolic flux control and alterations during light reduction ?
-
 ?

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
-
-

Purification (Commentary)

Purification (Comment) Organism
preparation of microsomal fraction Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
endosperm enzyme activity varies according to developmental stage of the endosperm, overview Hordeum vulgare
-
additional information no activity in mature seeds, no correlation between enzyme activity and xylan content in cell walls of different cultivars Hordeum vulgare
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000075 0.0004 xylan polymers Hordeum vulgare
0.002
-
 xyloseoligosaccharides Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity Hordeum vulgare ?
-
 ?
additional information metabolic flux control and alterations during light reduction Hordeum vulgare ?
-
 ?
pyridylaminated beta-(1,4)-xylotriose + UDP-D-xylose
-
 Hordeum vulgare pyridylaminated beta-(1,4)-xylotetraose + UDP
-
 ?
UDP-D-xylose + (1,4-beta-D-xylan)7
-
 Hordeum vulgare UDP + (1,4-beta-D-xylan)8
-
 ?
UDP-D-xylose + (1,4-beta-D-xylan)8
-
 Hordeum vulgare UDP + (1,4-beta-D-xylan)9
-
 ?
UDP-D-xylose + (1,4-beta-D-xylan)9
-
 Hordeum vulgare UDP + (1,4-beta-D-xylan)10
-
 ?
UDP-D-xylose + (1,4-beta-D-xylan)n substrate are e.g. higher xylooligosaccharide or xylans from different sources, chain elongation by formation of beta(1,4)-linkages, xylooligosaccharide substrates can be pyrimidylaminated or derivatized with 2-aminopyridine at their reducing ends and still serve as substrates, substrate can contain single 4-O-methyl-glucuronic acid residues Hordeum vulgare UDP + (1,4-beta-D-xylan)n+1
-
 ?
UDP-D-xylose + 4-O-methyl-glucuronoxylan
-
 Hordeum vulgare UDP + ?
-
 ?
UDP-D-xylose + arabinoxylan
-
 Hordeum vulgare UDP + ?
-
 ?

Synonyms

Synonyms Comment Organism
beta-(1,4)-xylosyltransferase
-
 Hordeum vulgare
XylTase
-
 Hordeum vulgare

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
-
 Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Hordeum vulgare