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Literature summary for 2.4.2.2 extracted from

  • Vande Voorde, J.; Gago, F.; Vrancken, K.; Liekens, S.; Balzarini, J.
    Characterization of pyrimidine nucleoside phosphorylase of Mycoplasma hyorhinis: implications for the clinical efficacy of nucleoside analogues (2012), Biochem. J., 445, 113-123.
    View publication on PubMed
Search for more literature for 2.4.2.2

Application

Application Comment Organism
medicine the cytostatic and antiviral activity of pyrimidine nucleoside analogues is markedly decreased by a Mycoplasma hyorhinis infection and the phosphorolytic activity of the mycoplasmas is responsible for this Mycoplasma hyorhinis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycoplasma hyorhinis

Inhibitors

Inhibitors Comment Organism Structure
5-benzylacyclouridine
-
 Mycoplasma hyorhinis
5-chloro-6-(1-[2-iminopyrrolidinyl]methyl)uracil
-
 Mycoplasma hyorhinis
7-deazaxanthine
-
 Mycoplasma hyorhinis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.047
-
 5-fluorouridine pH 7.6, 37°C Mycoplasma hyorhinis
0.069
-
 5-iodouridine pH 7.6, 37°C Mycoplasma hyorhinis
0.092
-
 uridine pH 7.6, 37°C Mycoplasma hyorhinis
0.144
-
 5-iododeoxyuridine pH 7.6, 37°C Mycoplasma hyorhinis
0.169
-
 5-fluorodeoxyuridine pH 7.6, 37°C Mycoplasma hyorhinis
0.388
-
 phosphate cosubstrate uridine, pH 7.6, 37°C Mycoplasma hyorhinis
0.473
-
 thymidine pH 7.6, 37°C Mycoplasma hyorhinis
0.578
-
 deoxyuridine pH 7.6, 37°C Mycoplasma hyorhinis
0.797
-
 phosphate cosubstrate deoxythymidine, pH 7.6, 37°C Mycoplasma hyorhinis

Organism

Organism UniProt Comment Textmining
Mycoplasma hyorhinis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-bromodeoxyuridine + phosphate
-
 Mycoplasma hyorhinis 5-bromouracil + 2-deoxy-D-ribose 1-phosphate
-
 ?
5-chlorodeoxyuridine + phosphate
-
 Mycoplasma hyorhinis 5-chlorouracil + 2-deoxy-D-ribose 1-phosphate
-
 ?
5-fluorodeoxyuridine + phosphate
-
 Mycoplasma hyorhinis 5-fluorouracil + 2-deoxy-D-ribose 1-phosphate
-
 ?
5-fluorouridine + phosphate
-
 Mycoplasma hyorhinis 5-fluorouracil + alpha-D-ribose 1-phosphate
-
 ?
5-iododeoxyuridine + phosphate
-
 Mycoplasma hyorhinis 5-iodouracil + 2-deoxy-D-ribose 1-phosphate
-
 ?
5-iodouridine + phosphate
-
 Mycoplasma hyorhinis 5-iodouracil + alpha-D-ribose 1-phosphate
-
 ?
5-trifluoromethyl-2'-deoxyuridine + phosphate
-
 Mycoplasma hyorhinis 5-trifluoromethyluracil + 2-deoxy-D-ribose 1-phosphate
-
 ?
deoxyuridine + phosphate
-
 Mycoplasma hyorhinis uracil + 2-deoxy-D-ribose 1-phosphate
-
 ?
additional information no substrate: cytidine, deoxycytidine, 2',2'-difluoro-2'-deoxycytidine, 2',2'-difluoro-2'-deoxyuridine, uracil arabinoside, thymine arabinoside, (E)-5-(2-bromovinyl)-deoxyuridine, azidothymidine, stavudine, 6-azauridine Mycoplasma hyorhinis ?
-
 ?
thymidine + phosphate
-
 Mycoplasma hyorhinis thymine + 2-deoxy-D-ribose 1-phosphate
-
 ?
uridine + phosphate prefered substrate Mycoplasma hyorhinis uracil + alpha-D-ribose 1-phosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1
-
 5-fluorouridine pH 7.6, 37°C Mycoplasma hyorhinis
6.4
-
 5-iodouridine pH 7.6, 37°C Mycoplasma hyorhinis
8.5
-
 uridine pH 7.6, 37°C Mycoplasma hyorhinis
8.6
-
 phosphate cosubstrate uridine, pH 7.6, 37°C Mycoplasma hyorhinis
9.5
-
 5-iododeoxyuridine pH 7.6, 37°C Mycoplasma hyorhinis
11.9
-
 5-fluorodeoxyuridine pH 7.6, 37°C Mycoplasma hyorhinis
17.5
-
 phosphate cosubstrate deoxythymidine, pH 7.6, 37°C Mycoplasma hyorhinis
21.6
-
 thymidine pH 7.6, 37°C Mycoplasma hyorhinis
24.6
-
 deoxyuridine pH 7.6, 37°C Mycoplasma hyorhinis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000003
-
 substrate uridine, pH 7.6, 37°C Mycoplasma hyorhinis 5-benzylacyclouridine
0.000005
-
 substrate deoxythymidine, pH 7.6, 37°C Mycoplasma hyorhinis 5-benzylacyclouridine
0.03
-
 substrate deoxythymidine, pH 7.6, 37°C Mycoplasma hyorhinis 7-deazaxanthine
0.06
-
 substrate uridine, pH 7.6, 37°C Mycoplasma hyorhinis 7-deazaxanthine
0.5
-
 substrate deoxythymidine, pH 7.6, 37°C Mycoplasma hyorhinis 5-chloro-6-(1-[2-iminopyrrolidinyl]methyl)uracil
0.5
-
 substrate uridine, pH 7.6, 37°C Mycoplasma hyorhinis 5-chloro-6-(1-[2-iminopyrrolidinyl]methyl)uracil

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
22
-
 phosphate cosubstrate deoxythymidine, pH 7.6, 37°C Mycoplasma hyorhinis
22
-
 phosphate cosubstrate uridine, pH 7.6, 37°C Mycoplasma hyorhinis
43
-
 deoxyuridine pH 7.6, 37°C Mycoplasma hyorhinis
46
-
 thymidine pH 7.6, 37°C Mycoplasma hyorhinis
66
-
 5-iododeoxyuridine pH 7.6, 37°C Mycoplasma hyorhinis
70
-
 5-fluorodeoxyuridine pH 7.6, 37°C Mycoplasma hyorhinis
92
-
 uridine pH 7.6, 37°C Mycoplasma hyorhinis
93
-
 5-iodouridine pH 7.6, 37°C Mycoplasma hyorhinis
130
-
 5-fluorouridine pH 7.6, 37°C Mycoplasma hyorhinis