Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
H175A | inactive | Mycobacterium tuberculosis |
K172A | inactive | Mycobacterium tuberculosis |
R105A | inactive | Mycobacterium tuberculosis |
R139A | inactive | Mycobacterium tuberculosis |
R162A | inactive | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Pyrazinamide | - |
Mycobacterium tuberculosis | |
pyrazinoic acid | - |
Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
quinolinic acid | at pH 7.2 and 37°C | Mycobacterium tuberculosis | |
0.39 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.2 and 37°C | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 12% activity in the presence of Mn2+ compared to Mg2+ | Mycobacterium tuberculosis | |
Fe2+ | 6% activity in the presence of Mn2+ compared to Mg2+ | Mycobacterium tuberculosis | |
Mg2+ | the enzyme requires bivalent metal ions and its activity is highest in the presence of Mg2+ | Mycobacterium tuberculosis | |
Mn2+ | 27% activity in the presence of Mn2+ compared to Mg2+ | Mycobacterium tuberculosis | |
additional information | not stimulated by Ca2+, Zn2+, and Cu2+ | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
gel filtration | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
quinolinic acid + 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis | - |
nicotinic acid mononucleotide + diphosphate + CO2 | - |
? | |
quinolinic acid + 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis H37Rv | - |
nicotinic acid mononucleotide + diphosphate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WJJ7 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WJJ7 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex 200 gel filtration | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.2 | - |
cell extract, at pH 7.2 and 37°C | Mycobacterium tuberculosis |
1.2 | - |
after 6.4fold purification, at pH 7.2 and 37°C | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
quinolinic acid + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis | nicotinic acid mononucleotide + diphosphate + CO2 | - |
? | |
quinolinic acid + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis H37Rv | nicotinic acid mononucleotide + diphosphate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NadC | - |
Mycobacterium tuberculosis |
QAPRTase | - |
Mycobacterium tuberculosis |
quinolinic acid phosphoribosyltransferase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
quinolinic acid | at pH 7.2 and 37°C | Mycobacterium tuberculosis | |
0.14 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.2 and 37°C | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.2 | - |
- |
Mycobacterium tuberculosis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.2 | 11 | activity cannot be detected at pH values below 5.2 and above 11.0 | Mycobacterium tuberculosis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.38 | - |
at pH 7.2 and 37°C | Mycobacterium tuberculosis | Pyrazinamide | |
1.37 | - |
at pH 6.2 and 37°C | Mycobacterium tuberculosis | Pyrazinamide | |
3.45 | - |
at pH 7.2 and 37°C | Mycobacterium tuberculosis | pyrazinoic acid | |
20 | - |
IC50 above 20 mM, at pH 6.2 and 37°C | Mycobacterium tuberculosis | pyrazinoic acid |