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Literature summary for 2.4.2.19 extracted from
- Biochemical characterization of quinolinic acid phosphoribosyltransferase from Mycobacterium tuberculosis H37Rv and inhibition of its activity by pyrazinamide (2014), PLoS ONE, 9, e100062.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H175A | inactive | Mycobacterium tuberculosis |
| K172A | inactive | Mycobacterium tuberculosis |
| R105A | inactive | Mycobacterium tuberculosis |
| R139A | inactive | Mycobacterium tuberculosis |
| R162A | inactive | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Pyrazinamide | - |
Mycobacterium tuberculosis |  |
| pyrazinoic acid | - |
Mycobacterium tuberculosis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
quinolinic acid | at pH 7.2 and 37°C | Mycobacterium tuberculosis | |
| 0.39 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.2 and 37°C | Mycobacterium tuberculosis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 12% activity in the presence of Mn2+ compared to Mg2+ | Mycobacterium tuberculosis | |
| Fe2+ | 6% activity in the presence of Mn2+ compared to Mg2+ | Mycobacterium tuberculosis | |
| Mg2+ | the enzyme requires bivalent metal ions and its activity is highest in the presence of Mg2+ | Mycobacterium tuberculosis | |
| Mn2+ | 27% activity in the presence of Mn2+ compared to Mg2+ | Mycobacterium tuberculosis | |
| additional information | not stimulated by Ca2+, Zn2+, and Cu2+ | Mycobacterium tuberculosis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
gel filtration | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| quinolinic acid + 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis | - |
nicotinic acid mononucleotide + diphosphate + CO2 | - |
? | |
| quinolinic acid + 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis H37Rv | - |
nicotinic acid mononucleotide + diphosphate + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | P9WJJ7 | - |
- |
| Mycobacterium tuberculosis H37Rv | P9WJJ7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap column chromatography and Superdex 200 gel filtration | Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.2 | - |
cell extract, at pH 7.2 and 37°C | Mycobacterium tuberculosis |
| 1.2 | - |
after 6.4fold purification, at pH 7.2 and 37°C | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| quinolinic acid + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis | nicotinic acid mononucleotide + diphosphate + CO2 | - |
? | |
| quinolinic acid + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis H37Rv | nicotinic acid mononucleotide + diphosphate + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NadC | - |
Mycobacterium tuberculosis |
| QAPRTase | - |
Mycobacterium tuberculosis |
| quinolinic acid phosphoribosyltransferase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
quinolinic acid | at pH 7.2 and 37°C | Mycobacterium tuberculosis | |
| 0.14 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 7.2 and 37°C | Mycobacterium tuberculosis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.2 | - |
- |
Mycobacterium tuberculosis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | 11 | activity cannot be detected at pH values below 5.2 and above 11.0 | Mycobacterium tuberculosis |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.38 | - |
at pH 7.2 and 37°C | Mycobacterium tuberculosis | Pyrazinamide | |
| 1.37 | - |
at pH 6.2 and 37°C | Mycobacterium tuberculosis | Pyrazinamide | |
| 3.45 | - |
at pH 7.2 and 37°C | Mycobacterium tuberculosis | pyrazinoic acid | |
| 20 | - |
IC50 above 20 mM, at pH 6.2 and 37°C | Mycobacterium tuberculosis | pyrazinoic acid | |
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