Literature summary for 2.4.2.19 extracted from

di Luccio, E.; Wilson, D.K.
Comprehensive X-ray structural studies of the quinolinate phosphoribosyl transferase (BNA6) from Saccharomyces cerevisiae (2008), Biochemistry, 47, 4039-4050.

Cloned(Commentary)

Cloned (Comment)	Organism
into plasmid pTYB12 and expressed in Escherichia coli BL21	Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment)	Organism
apo, quinolinate-bound, 5-phospho-alpha-D-ribose 1-diphosphate-bound, and phthalate-bound forms. Crystallized at room temperature by the hanging drop vapor diffusion method. Both apo and holo crystals belong to space group R32 (alpha = 90°, beta = 90°, and gamma = 120°). One molecule per asymmetric unit except in QAPRTase holophthalate crystals where two molecules are found. The unit cell dimensions are: a = b = 154.9 A and c = 68.9 A (apo), a = b = 154.8 A and c = 68.6 A (holoquinolinate), a = b = 154.9 A and c = 70.6 A (holo-5-phospho-alpha-D-ribose 1-diphosphate), a = b = 155.5 A and c = 121.1 A (holophthalate), a = b = 154.7 A and c = 69.3 A (holophthalate-5-phospho-alpha-D-ribose 1-diphosphate)	Saccharomyces cerevisiae

Crystallization (Comment)

Organism

apo, quinolinate-bound, 5-phospho-alpha-D-ribose 1-diphosphate-bound, and phthalate-bound forms. Crystallized at room temperature by the hanging drop vapor diffusion method. Both apo and holo crystals belong to space group R32 (alpha = 90°, beta = 90°, and gamma = 120°). One molecule per asymmetric unit except in QAPRTase holophthalate crystals where two molecules are found. The unit cell dimensions are: a = b = 154.9 A and c = 68.9 A (apo), a = b = 154.8 A and c = 68.6 A (holoquinolinate), a = b = 154.9 A and c = 70.6 A (holo-5-phospho-alpha-D-ribose 1-diphosphate), a = b = 155.5 A and c = 121.1 A (holophthalate), a = b = 154.7 A and c = 69.3 A (holophthalate-5-phospho-alpha-D-ribose 1-diphosphate)

Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
Phthalate	-	Saccharomyces cerevisiae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
191000	-	6 * 191000, dynamic light scattering analysis	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P43619	-	-

Purification (Commentary)

Purification (Comment)	Organism
by anion exchange chromatography	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
quinolinic acid + 5-phospho-alpha-D-ribose 1-diphosphate	-	Saccharomyces cerevisiae	nicotinic acid mononucleotide + diphosphate + CO2	-	?

Subunits

Subunits	Comment	Organism
hexamer	6 * 191000, dynamic light scattering analysis	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
BNA6	-	Saccharomyces cerevisiae
QAPRTase	-	Saccharomyces cerevisiae
quinolinate phosphoribosyl transferase	-	Saccharomyces cerevisiae