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Literature summary for 2.4.2.19 extracted from

  • Bhatia, R.; calvo, K.C.
    The sequencing, expression, purification, and steady-state kinetic analysis of quinolinate phosphoribosyl transferase from Escherichia coli (1996), Arch. Biochem. Biophys., 325, 270-278.
    View publication on PubMed
Search for more literature for 2.4.2.19

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
D-fructose-1,6-diphosphate competitive with respect to 5-phosphoribosyl-1-diphosphate and noncompetitive with respect to quinolinate Escherichia coli
diphosphate noncompetitive with respect to both 5-phosphoribosyl-1-diphosphate and quinolinate Escherichia coli
nicotinate mononucleotide competitive with respect to 5-phosphoribosyl-1-diphosphate Escherichia coli
Phthalic acid dead-end inhibitor, competitive with respect to quinolinate, uncompetitive with respect to 5-phosphoribosyl-1-diphosphate Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0064
-
 quinolinate
-
 Escherichia coli
0.0156
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 2 * 36000, SDS-PAGE Escherichia coli
70000
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P30011
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
beta-nicotinate D-ribonucleotide + diphosphate + CO2 = pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate ordered binding mechanism Escherichia coli
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
4°C, stable for 1 month Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Escherichia coli nicotinate D-ribonucleotide + diphosphate + CO2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 36000, SDS-PAGE Escherichia coli