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Literature summary for 2.4.2.18 extracted from

  • Lambrecht, J.A.; Downs, D.M.
    Anthranilate phosphoribosyl transferase (TrpD) generates phosphoribosylamine for thiamine synthesis from enamines and phosphoribosyl pyrophosphate (2013), ACS Chem. Biol., 8, 242-248.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged protein expressed in Escherichia coli Salmonella enterica

Protein Variants

Protein Variants Comment Organism
D282A mutant enzyme does not generate sufficient phosphoribosyl amine to allow growth in the absence of thiamine, mutant enzyme does synthesize sufficient tryptophan to allow growth (in vivo analysis using the Salmonella enterica strain DM9813 (purF gnd ridA trpD, auxotrophic requirement for purines, thiamine and tryptophan)) Salmonella enterica
G308L mutant enzyme does not generate sufficient phosphoribosyl amine to allow growth in the absence of thiamine, mutant enzyme does synthesize sufficient tryptophan to allow growth (in vivo analysis using the Salmonella enterica strain DM9813 (purF gnd ridA trpD, auxotrophic requirement for purines, thiamine and tryptophan)) Salmonella enterica
H307A mutant enzyme does not generate sufficient phosphoribosyl amine to allow growth in the absence of thiamine, mutant enzyme does synthesize sufficient tryptophan to allow growth (in vivo analysis using the Salmonella enterica strain DM9813 (purF gnd ridA trpD, auxotrophic requirement for purines, thiamine and tryptophan)) Salmonella enterica
K306A mutant enzyme does not generate sufficient phosphoribosyl amine to allow growth in the absence of thiamine (in vivo analysis using the Salmonella enterica strain DM9813 (purF gnd ridA trpD, auxotrophic requirement for purines, thiamine and tryptophan)) Salmonella enterica
N309A mutant enzyme does not generate sufficient phosphoribosyl amine to allow growth in the absence of thiamine, mutant enzyme does synthesize sufficient tryptophan to allow growth (in vivo analysis using the Salmonella enterica strain DM9813 (purF gnd ridA trpD, auxotrophic requirement for purines, thiamine and tryptophan)) Salmonella enterica
R364A mutant enzyme does not generate sufficient phosphoribosyl amine to allow growth in the absence of thiamine (in vivo analysis using the Salmonella enterica strain DM9813 (purF gnd ridA trpD, auxotrophic requirement for purines, thiamine and tryptophan)) Salmonella enterica
T279A mutant enzyme does not generate sufficient phosphoribosyl amine to allow growth in the absence of thiamine, mutant enzyme does synthesize sufficient tryptophan to allow growth (in vivo analysis using the Salmonella enterica strain DM9813 (purF gnd ridA trpD, auxotrophic requirement for purines, thiamine and tryptophan)) Salmonella enterica

Organism

Organism UniProt Comment Textmining
Salmonella enterica
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-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Ni2+) Salmonella enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminocrotonate + 5-phospho-alpha-D-ribose 1-diphosphate
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Salmonella enterica N-(5-phospho-D-ribosyl)-2-aminocrotonate + diphosphate reaction product predicted to be a phosphoribosyl-enamine adduct, which breaks down directly into phosphoribosyl amine and 2-ketobutyrate (a reaction mediated by TrpD or non-enzymatic), or which breaks down non-enzymatically into ribose 5'-phosphate, 2-ketobutyrate and NH3. Ribose 5'-phosphate and NH3 combine non-enzymatically to form phosphoribosyl amine ?
anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate also 4- and 5-carbon enamines (2-aminocrotonate, 2-amino-2-pentenoate, also 2-aminoacrylate) serve as substrates, 2-aminobutyrate is not used as substrate Salmonella enterica N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
?

Synonyms

Synonyms Comment Organism
anthranilate phosphoribosyl transferase bifunctional enzyme EC 4.1.3.27, 2.4.2.18 Salmonella enterica
Trp D
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Salmonella enterica

General Information

General Information Comment Organism
physiological function tryptophan biosynthetic pathway Salmonella enterica