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Literature summary for 2.4.2.18 extracted from

  • Bode, R.; Birnbaum, D.
    Enzymes of the aromatic amino acid biosynthesis in Hansenula henricii: anthranilate phosphoribosylpyrophosphate-phosphoribosyltransferase (E.C.2.4.2.18) (1978), Z. Allg. Mikrobiol., 18, 559-566.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
3-Hydroxyanthranilate competitive Ogataea henricii
anthranilate substrate inhibition above 0.008 mM Ogataea henricii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0046
-
anthranilate
-
Ogataea henricii
0.88
-
5-phospho-alpha-D-ribose 1-diphosphate
-
Ogataea henricii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Ogataea henricii
Mg2+ Km: 0.056 mM Ogataea henricii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
gel filtration Ogataea henricii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate Ogataea henricii enzyme of tryptophan biosynthesis N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Ogataea henricii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Ogataea henricii

Reaction

Reaction Comment Organism Reaction ID
N-(5-phospho-D-ribosyl)-anthranilate + diphosphate = anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate reaction mechanism Ogataea henricii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.4
-
-
Ogataea henricii

Storage Stability

Storage Stability Organism
-25°C, 500 mM Tris-HCl, pH 7.5, 2 mM EDTA, 2 mM MgCl2, 5% loss of activity after 2 weeks Ogataea henricii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate
-
Ogataea henricii N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
?
anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate enzyme of tryptophan biosynthesis Ogataea henricii N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
?

Subunits

Subunits Comment Organism
More no aggregation with other enzymes Ogataea henricii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Ogataea henricii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 7.7
-
Ogataea henricii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.012
-
3-Hydroxyanthranilate
-
Ogataea henricii
0.03
-
anthranilate
-
Ogataea henricii